Studies on Fungal and Bacterial Endophytes of Soybean (Glycine max (L.) Merrill) Against the Soilborne Pathogens
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Date
0200
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Agricultural Sciences, Dharwad
Abstract
A total of 30 fungal and 30 bacterial endophytes were isolated from healthy
soybean plants during kharif 2017 from major soybean growing areas of northern
Karnataka viz., Belagavi, Haveri, Bidar, Dharwad and parts of Maharashtra viz.,
Kolhapur and Sangli districts. Maximum number of endophytes were obtained from
Belagavi district (21) followed by Dharwad district (15). Mean maximum endophyte
association was observed in irrigated condition (1.38 for fungal and 1.63 for bacterial
endophytes), black soil (1.31 for fungal and 1.46 for bacterial) and at vegetative stage
(1.73 for fungal and 1.27 for bacterial).
In dual culture method of screening, the endophytes RF-BV-3, SF-DM-8,
RB-KK-6, SB-BS-6 and LB-BU-1 were effective against Sclerotium rolfsii and the
isolates SF-DM-8, SB-DG-11 and LB-BiN-8 were effective against Rhizoctonia
bataticola. The effective endophytes against Fusarium oxysporum were RF-BV-3,
SF-BV-3, SF-DM-8, SF-DS-10, LF-HH-5, LF-DM-10, LF-DD-13, LF-KK-14,
RB-HS-1, SB-BiJ-9, LB-BU-1 and LB-BV-2. Based on cultural, morphological and
molecular methods, the effective fungal endophytes were identified as Daldinia
eschscholtzi (RF-BV-3), Fusarium solani (SF-BV-3 and LF-KK-14), Neofusicoccum
parvum (SF-DM-8), Diaporthe phaseolorum (SF-DS-10 and LF-HH-5), Phomopsis sp.
(LF-DM-10) and Colletotrichum aenigma (LF-DD-13). The effective bacterial
endophytes were identified as Acinetobacter sp. (RB-HS-1), Alcaligenes faecalis
(RB-KK-6), Stenotrophomonas sp. (SB-BiJ-9), Bacillus pumilus (SB-DG-11 and
LB-BiN-8), Paenalcaligenes sp. (LB-BU-1), Bacillus cereus (SB-BS-6) and
Brevibacillus sp. (LB-BV-2).
Under glasshouse studies, SF-DM-8 was effective against all the three pathogens
(less disease incidence of 21.88 % against S. rolfsii, 27.50 % against R. bataticola and
25.00 % against F. oxysporum) and showed enhanced growth promotion activity
(increased plant height, leaflet number etc.) with maximum disease incidence of 100
per cent in untreated check. The endophyte RB-KK-6 was effective against S. rolfsii
with less disease incidence (31.25 %). The endophytes RF-BV-3 and SB-BiJ-9 were
effective against F. oxysporum with minimum disease incidence of 16.67 per cent.