Characterization of bubaline epididymal spermatozoa stored at 5°c in-situ and their storage resistance in modified citrate dilutors
| dc.contributor.advisor | Gupta, H.P. | |
| dc.contributor.author | Rana, Ramkrishan | |
| dc.date.accessioned | 2018-08-23T08:22:19Z | |
| dc.date.available | 2018-08-23T08:22:19Z | |
| dc.date.issued | 2017-08 | |
| dc.description.abstract | The present study was undertaken to determine the testicular biometry of slaughtered buffalo bull testis and its correlation with sperm production, to find out the improvement in keeping quality of epididymal spermatozoa in egg yolk citrate diluter modified with alanine, cysteine and glutamine and to evaluate the effect of amino acid on structural aspect and storage resistance of spermatozoa through initial motility, live count, percent abnormality and HOS reacted spermatozoa. The 48 testicles from slaughtered buffalo bulls were collected from slaughter house, and divided into 4 groups each containing 12 testicles; G1 (semen collection at 0 hours of testicle storage), G2 (semen collection at 24 hours of testicle storage), G3 (semen collection at 48 hours of testicle storage) and G4 (semen collection at 72 hours of testicle storage). The testicles were processed on the same day and evaluated for morphological traits and seminal characteristics. The epididymal semen was collected from refrigeration storage (5ºC) of testicles at 0, 24, 48 and 72 hours and further percent initial motility, livability, abnormality and HOS positive spermatozoa was evaluated . The biometric testicular parameters (testicular length with epididymis and without epididymis, circumference, diameter, weight, volume, caput length, width and weight, corpus length, width and weight, cauda length, width and weight) were measured and significant (P<0.05) difference between left and right testis was observed The significant (P<0.05) variation in seminal attribute was observed among testicles as well as among the groups. The post thaw semen quality was assessed 24, 48 and 72 hour after refrigeration of epididymal semen. A significant improvement in post thaw seminal characteristics and HOS reactive sperm was observed among the treatments. Incorporation of glutamine @ dose of 25 mM and cysteine @ 5 mM in diluter resulted in maximum increase in post thaw % motility, live sperm, post thaw HOS positive sperm as compared to alanine and control group. Glutamine and cysteine, promising semen additive which can be used further to improve the quality of refrigerated thawed semen. The data was processed using statistical analysis programme. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810067088 | |
| dc.keywords | Characterization of bubaline epididymal spermatozoa stored at 5°c in-situ and their storage resistance in modified citrate dilutors | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 137 | en_US |
| dc.research.problem | Buffaloes | en_US |
| dc.sub | Veterinary Gynaecology and Obstetrics | en_US |
| dc.subject | null | en_US |
| dc.theme | Spermatozoa | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Characterization of bubaline epididymal spermatozoa stored at 5°c in-situ and their storage resistance in modified citrate dilutors | en_US |
| dc.type | Thesis | en_US |