DEVELOPMENT AND CHARACTERIZATION OF ESTSSR MARKERS IN Rubus ellipticus SMITH AND THEIR TRANSFERABILITY IN RELATED SPECIES/GENERA

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dc.contributor.advisorKAUR, RRAJINDER
dc.contributor.authorSAMRITI
dc.date.accessioned2018-03-22T07:50:17Z
dc.date.available2018-03-22T07:50:17Z
dc.date.issued2018-03
dc.description.abstractABSTRACT Rubus ellipticus Smith. commonly known as ‘Yellow Himalayan raspberry’ is an important member of Rosaceae family with antioxidant and antibacterial properties. In the present study EST-SSR markers were developed for R. ellipticus and were used for polymorphism/transferability studies. EST sequences of R. ellipticus were generated from cDNA clones using Paired-End sequencing method on NextSeq 500. The raw data were assembled using DNASTAR (https://www.dnastar.com/ ) software. A total of 7777 EST sequences for R. ellipticus were obtained. ESTs containing SSR motifs were extracted out using an online tool, MISA (http://pgrc.ipk-gatersleben.de/misa). 704 ESTs were used for SSR extraction. SSR primers were designed from the SSR containing EST sequences using Websat software (http:/www.wsmartins.net/websat) and 68 randomly selected primer pairs were custom synthesized. Polymorphism study was carried out using 68 SSR primer pairs among 21 collections of Rubus ellipticus and 31 members of Rosaceae family. For polymorphism studies, DNA was isolated from young leaves using CTAB method (Doyle and Doyle, 1987). The EST-SSR primers revealed 95.3% and 93.6% polymorphism in diversity and cross transferability analysis. Jaccard’s similarity matrix was developed and dendrograms were generated using NTSYSpc ver.2.02h to establish the percent similarity among the 21 collections of Rubus ellipticus and 31 members of Rosaceae family. With EST-SSR primers, two clusters were obtained. Maximum similarity i.e 69% was found between ‘Kharkog-1’ and ‘Majhgaon’ of Rubus ellipticus and 87 % betweenn‘High and magic’ and ‘Chaubattia ambrose’ of Rosaceae family. Thus EST-SSRs used in the present study revealed a high level of polymorphism in the 21 colllections of Rubus ellipticus and 31 members of Rosaceae family, revealing their efficiency in fingerprinting analysis.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810042359
dc.keywordsRubus ellipticus Smith.,Yellow Himalayan raspberry,EST-SSR markers,polymorphism/transferabilityen_US
dc.language.isoenen_US
dc.pages98+IIen_US
dc.publisherUHF,NAUNIen_US
dc.subBiotechnology and Molecular Biology
dc.subjectnullen_US
dc.themeRubus ellipticus Smith.,Yellow Himalayan raspberry,EST-SSR markers,polymorphism/transferabilityen_US
dc.these.typePh.Den_US
dc.titleDEVELOPMENT AND CHARACTERIZATION OF ESTSSR MARKERS IN Rubus ellipticus SMITH AND THEIR TRANSFERABILITY IN RELATED SPECIES/GENERAen_US
dc.typeThesisen_US
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