IDENTIFICATION OF GENOTYPES AND TAGGING OF AVAILABLE SSR MARKERS LINKED TO BCMV (BEAN COMMON MOSAIC VIRUS) RESISTANCE IN EARLY SEGREGATING GENERATION OF COWPEA [Vigna unguiculata (L.) Walp.] AND EVALUATION OF NATURE OF SEED TRANSMISSION
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Date
2017-07-27
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
BCMV has been found to infect large number of crop plants including cowpea
and cause substantial yield loss. In this study the reaction of the segregating population
of cowpea of selected crosses viz., IC39916 x IT38956-1 and IC39916 x KBC-2 were
evaluated to identify resistant lines for further advancement. Further the nature of seed
transmission of BCMV was investigated. Out of 200 plants of each cross of F2, 80
plants of cross IC39916 x IT38956-1 and 76 plants of cross IC39916 x KBC-2 showed
resistant reaction at 60 DAS. The 2 test results indicated the involvement of
complementary gene interaction in both the crosses for the disease reaction. Major yield
parameters like number of pods and seed weight per plant of both the crosses showed
the involvement of large number of genes with complementary epistasis while average
length of the pods showed duplicate gene interaction. Higher estimates of PCV for
number of pods and seed weight per plant showed high variability whereas average pod
length showed low variability. Among the Simple Sequence Repeats (SSR) markers
evaluated (M15, M135, M80 and Y96), the marker Y96 showed polymorphism for
disease reaction for parents (IC39916, IT 38956-1 and KBC-2) and F2 population from
both the crosses. The BCMV upon DAS-ELISA was found to be internally seed borne
in nature. The virus was detected in embryo, cotyledon but not in the seed coat with
varied concentrations in embryo and cotyledon, in both C-152 and KBC-2 varieties.
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