Seroprevalence and Restriction enzyme analysis of Egg Drop Syndrome virus

dc.contributor.advisorKrishnan Nair, G
dc.contributor.authorPriya, P M
dc.contributor.authorKAU
dc.date.accessioned2019-05-25T06:42:26Z
dc.date.available2019-05-25T06:42:26Z
dc.date.issued2000
dc.descriptionPGen_US
dc.description.abstractIn the present study, seroprevalence of EDS-76 was conducted in five districts of Kerala in duck and' chicken flocks using HI and ELISA. Out of 322 chicken and 281 duck sera samples screened, an overall incidence of 14.91 per cent and 26.69 per cent respectively were recorded. The high proportion of birds showing antibodies to EDS-76 reveals that the infection is widespread in Kerala and may be the major etiological factor associated with drop in egg production in poultry. Among the two serological tests namely, HI and ELISA employed for the detection of EDS-76 viral antibody, HI was found to be simple, sensitive and reliable. It is concluded that HI test could be used for the detection of EDS-76 infection in poultry flocks. Restriction DNA fingerprinting of the three indigenous strains were carried out in conjunction with the reference strain to check for any genetic variation between the strains. Comparison of the DNA fingerprint of all the four strains digested with restriction endonucleases BamHI, EcoRI and HindIII revealed identical banding pattern thereby conforming the genetic similarity of the strains.en_US
dc.identifier.citation171781en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810104568
dc.keywordsMicrobiology, egg drop syndrome virus, EDS 76 virusen_US
dc.language.isoenen_US
dc.publisherDepartment of Microbiology, College of Veterinary and Animal Sciences, Mannuthyen_US
dc.subMicrobiologyen_US
dc.subjectnullen_US
dc.themeEnzyme analysis of egg drop syndrome virusen_US
dc.these.typeM.V.Sc.en_US
dc.titleSeroprevalence and Restriction enzyme analysis of Egg Drop Syndrome virusen_US
dc.typeThesisen_US
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