Seroprevalence and Restriction enzyme analysis of Egg Drop Syndrome virus
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Date
2000
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Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy
Abstract
In the present study, seroprevalence of EDS-76 was
conducted in five districts of Kerala in duck and'
chicken flocks using HI and ELISA. Out of 322 chicken
and 281 duck sera samples screened,
an overall incidence of 14.91 per cent and 26.69 per cent
respectively were recorded. The high proportion of birds showing antibodies to EDS-76 reveals that the infection is widespread in Kerala and may be the major etiological
factor associated with
drop in egg production in poultry.
Among the two serological tests namely, HI and
ELISA employed for the detection of EDS-76 viral
antibody, HI was found to be simple, sensitive and
reliable. It is concluded that HI test could be used
for the detection of EDS-76 infection in poultry
flocks.
Restriction DNA fingerprinting of the three indigenous strains were carried out in conjunction with
the reference strain to check for any genetic variation
between the strains. Comparison of the DNA fingerprint
of all the four strains digested with restriction
endonucleases BamHI, EcoRI and HindIII revealed identical banding pattern thereby conforming the genetic similarity of the strains.
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Citation
171781