Genetic Analysis for Growth and Oleoresin Traits of Chir pine (Pinus roxburghii Sargent)

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dc.contributor.advisorSingh, N.B.
dc.contributor.authorBhat, Sheeraz Saleem
dc.date.accessioned2017-06-03T11:11:55Z
dc.date.available2017-06-03T11:11:55Z
dc.date.issued2015
dc.description.abstractThe present investigations entitled “Genetic Analysis for Growth and Oleoresin Traits of Chir pine (Pinus roxburghii Sargent)” were carried out in the Research field and Laboratory of the Department of Tree Improvement and Genetic Resources, College of Forestry, Dr YSP University of Horticulture and Forestry, Nauni, Solan (HP) during the year 2010-2012, to: 1) evaluate different growth traits (height, diameter at breast height, bark thickness, crown height and crown length), needle characteristics (colour, length, thickness and number of stomata per mm of row), wood traits (moisture content, specific gravity and tracheid length) of different half sib chir pine progenies; 2) to carry out molecular characterization through RAPD, ISSR and SSR markers, and, 3) to carry out marker trait association for the aforesaid traits, besides the oleoresin yield of the different genotypes. All the traits varied significantly, with mean values height (18.08 m), diameter at breast height (34.76 cm), bark thickness (2.73 cm), crown height (7.80 m), crown length (10.38 m), needle length (20.78 cm), thickness (0.61 mm), number of stomata per mm of a row (9.71), wood moisture content (54.31%), specific gravity (0.41) and tracheid length (1.68 mm). Variability estimates, genetic parameters, correlation studies, principal component analysis and divergence studies exhibited high variability among all the half sib progenies. Characters which exhibited high heritability like crown height (H2=0.45), wood specific gravity (H2=0.66), needle thickness (H2=0.48), followed by higher genetic gain show additive genetic variation and can be exploited quiet well in advanced breeding programs, thus more effective in selecting the best genotypes for a trait. Genetic divergence was studied through cluster analysis and dendrograms. Molecular characterization through RAPD, ISSR and SSR revealed high polymorphism percentage (90.14, 94.84 and 100, respectively); with respective band size ranges 105-1490 bp, 198-2015 bp and 103-239 bp. Dendrogram based on these markers, individually as well as combined, were made for diversity analysis. Structural analysis was carried out for the population diversity studies, and for association mapping. Three clusters resulted for each marker, beside some genotypes as admixtures. Association mapping with different traits revealed 12 associations with RAPD markers, 6 associations with ISSR markers, 8 associations with SSR markers at P value (<0.05). Coefficient of determination, R2 value, ranged from 0.0757 for UBC841 with wood specific gravity to 0.1495 for NAD3-1 with crown length.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810017023
dc.language.isoenen_US
dc.publisherYSPUen_US
dc.subForestry
dc.subjectgenetics, genotypes, wood, biological phenomena, polymorphism, biological development, rapd, selection, phenotypes, yieldsen_US
dc.these.typePh.D
dc.titleGenetic Analysis for Growth and Oleoresin Traits of Chir pine (Pinus roxburghii Sargent)en_US
dc.typeThesisen_US
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