IN VITRO STUDIES ON MODULATION OF CONTRACTILE AND RELAXANT RESPONSES OF SHEEP TRACHEAL SMOOTH MUSCLE BY MORIN
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Date
2016-07
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
Morin (3,5,7,2,4 - pentahydroxyflavone), belongs to the flavonoid group of
polyphenolic compounds found in wood of old fustic, in osage orange, white mulberry, fig,
almond and sweet chestnut, and many other herbs and fruits used as herbal medicines.
Morin has been the subject of a number of experimental studies dealing with its
pharmacological activities, such as antioxidant properties, inhibition of lipid peroxidation,
anti cancer activity, anti-inflammatory activity, cardiovascular protection and also a prooxidant
action. Despite the enormous interest in flavonoids as potential therapeutic agents,
their biological activity mechanisms are poorly understood and largely unknown, but
different types of possible biochemical events are involved. In view of this, the present
study is designed to know the modulatory effect of morin hydrate on contractile and
relaxant responses of various pharmacological agents like acetylcholine and atropine,
histamine and cetirizine, KCl and nifedipine in sheep tracheal smooth muscle in vitro.
Mean EC50 values for contractile response of acetylcholine obtained in the absence
of morin hydrate were 3.38×10-7M (5×10-9M to 15×10-6M) and in presence of morin
hydrate it was increased to 6.02×10-7M (5×10-9M to 15×10-5M) with a significant right
shift. Mean EC50 values for contractile response of histamine obtained in the absence of
morin hydrate were 1.12×10-6M (5×10-9M to 30×10-5M) and in presence of morin hydrate
it was increased to 2.63×10-6M (5×10-9M to 30×10-5M) with a significant right shift.
Mean IC50 values for relaxant response of atropine, cetirizine and nifedipine in
comparison with morin hydrate in sheep tracheal smooth muscle were 2.23×10-8M (5×10-
9M to 5×10-6M), 3.46×10-8M (5×10-9M to 15×10-6M), 6.30×10-6M (5×10-9M to 30×10-
5M) and 6.30×10-7M (5×10-9M to 15×10-6M), 1.58×10-6M (5×10-9M to 5×10-5M),
2.04×10-5M (5×10-9M to 15×10-4M) respectively.
The study revealed that morin hydrate has relaxant effect on the sheep trachealis
smooth muscle in vitro.
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