STUDIES ON CHARACTERIZATION OF HYPER PHOSPHATE SOLUBILIZING MUTANT STRAIN OF Bacillus licheniformis
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Date
2016
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Abstract
ABSTRACT
Plant growth promoting rhizobacteria (PGPR) are root associated bacteria and benefit plants by providing growth promotion by a variety of mechanism. The present study was focused on hyper phosphate solubilization by mutagenesis of wild strain (WS) of B. licheniformis originally isolated from apple rhizosphere. Ultraviolet (UV) rays, Ethyl methanesulphonate (EMS) and Ethidium bromide (EtBr) mutagens were used to enhance the phosphate solubilizing ability of wild strain. Forty mutants were selected on the basis of phosphate solubilizing index (PSI) and change in colony morphology. Only one mutant PM7 with hyper phosphate solubilization was obtained after treating WS with UV rays for 100 minutes of exposure. Of all the mutants tested, PM7 was selected, as there was approximately 2 fold increase in P-solubilization over wild type. Mutant PM7 showed higher PSI together with higher IAA and siderophore production 43.00 µg/ml and 103.09 % SU, respectively. Mutant PM7 also showed high antifungal activity against Phytophthora capsici (64.44 % G.I), Fusarium oxysporum (62.30 % G.I) and Dematophora necatrix (65.12 % G.I) in comparison to WS. Thus, mutant PM7 was selected for net house trial on growth promotion of tomato. Remarkably, enhanced root/shoot parameters, P-uptake and available P content of soil at 50 % of recommended dose of tricalcium phosphate was recorded as compared to WS and control. The result obtained in the present study, indicate toward the great potential of hyper Psolubilization of mutant strain of Bacillus licheniformis to be developed as biofertilizers to enhance soil fertility and plant growth.
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inorganic acid salts, bacteria, mutants, planting, biological development, growth promoters, productivity, fungi, acidity, vegetative propagation, Plant growth promoting rhizobacteria (PGPR)