EPIZOOTIOLOGY OF MIXED INFECTION (FLACHERIE DISEASE) OF SILKWORM, Bombyx mori L. AND MOLECULAR CHARACTERIZATION OF ASSOCIATED BACTERIA
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Date
2016-10-10
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
The studies on “Epizootiology of mixed infection (flacherie disease) of
silkworm, Bombyx mori L. and molecular characterization of associated bacteria”
revealed that, there are ten bacterial isolates were isolated from different sources, only
three isolates were used for molecular identification through 16S rRNA gene and in vivo
study with BmNPV. As per the sequencing sof organisms, the isolated bacteria 1 had 95
percent similarity with Lysinibacillus sphaericus (LS), isolated bacteria 2 had 99 per cent
similarity with Alcaligence faecalis (AF) strain SDK 2 which was reported first time in
the infected silkworm. Isolated bacteria 3 had 99 per cent similarity with Bacillus subtilis
C11 (BS). Inoculation of three bacterial isolates to third, fourth and fifth instar revealed,
extended moulting and larval duration. In addition, ET50 for mortality and symptoms
expression days were recorded maximum of 10.98 (AF) and 13.34 (AF) followed by 8.66
(BS+LS) and 10.76 (BS+LS) and minimum was recorded in combination of 5.05
(N+LS+AF) and 6.50 days (N+BS+LS). In dual inoculation viz., LS+AF (56.10, 47.95
and 52.05 %), AF+LS (48.02, 40.10 and 59.90 %), BS+LS recorded 46.81, 40.43 and
59.57 per cent number of worms entered to spinning, effective rate of rearing and larval
mortality respectively. The BS+LS inoculated batches were recorded more cocoon
weight (1.20, 1.25 1.32 g), shell weight (0.19, 0.22, 0.26 g) and shell percentage (15.83,
17.60 and 19.69 %) in all the three instars where as, the minimum was in BS+LS+N
compared to control. It was clearly inferred that, the newly identified AF found to be less
pathogenic compared to in combination.
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