INDUCTION OF MALE STERILITY IN SILKWORM UZI FLY, Exorista bombycis (Louis) - A PARASITOID OF SILKWORM, Bombyx mori L.

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Date
2014-08-08
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University of Agricultural Sciences GKVK, Bangalore
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The investigations on the “Induction of male sterility in silkworm uzi fly, Exorista bombycis (Louis) - A parasitoid of silkworm, Bombyx mori L.” was carried out during 2011-2014 at the Department of Sericulture, University of Agricultural Sciences, GKVK in collaboration with National Center for Biological Sciences, Bangalore for gamma irradiation. The highest uzi fly emergence (89.79%), mating duration (1.56hr) and mating percentage (88.56 %) was recorded in male flies emerged from 8 day old irradiated pupae at 2kr compared to other treated batches. Whereas, the normal female flies mated with sterile males emerged from 8 day old irradiated pupae at 2kr recorded maximum preovipositional period (32hr) and fecundity (510). The maximum time taken for sclerotization (70, 65 and 63.54 min.) by male flies that emerged from 3 day old irradiated pupae at 1, 1.5 and 2kr respectively as compared to control. The minimum ovipositional period (3days), number of fertile eggs (110) and hatching percentage (24.81%) was recorded in normal females mated with sterile males emerged from 8 day old irradiated pupae at 2kr. Studies on field release of sterile male flies near rearing houses resulted in decreased hatching percentage (17.38%) compared to un released village. Mass production of uzi maggots in-vitro revealed that 2 mated females provided with 300 worms for oviposition resulted in maximum fecundity (657), maggots formed (562), pupae formed (544), flies emerged (534), male pupal weight (1.26 g), female pupal weight (0.96/g), male pupal length (0.91cm) and female pupal length (0.97cm) compared to 50 , 100 and 200 silkworms provided batches.
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