Molecular characterization of yellow mosaic virus on Pole bean (Phaseolus vulgaris L.) and its disease management
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Date
41482
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Yellow mosaic, a begomovirus is the most important disease of pole
bean. The complete coat protein gene sequences have been determined
and are comprised of approximately 1000 nucleotides, the
characterization of coat protein gene showed that, the Pole bean yellow
mosaic virus showed highest sequence identity (99.7%) with French bean
yellow mosaic virus (FbYMV) GKVK isolate. At amino acid level pole bean
yellow mosaic virus isolate has 100 per cent homology with FbYMV
GKVK isolate. The results obtained in the present study clearly indicates
that YMV infecting pole bean at Bangalore is closely related to FbYMV
GKVK isolate, rather than MYMV. The coat protein gene of Pole bean and
French bean yellow mosaic virus isolates were sequenced assembled and
its total length was determined as 920 bp. The actual length of coat
protein gene of YMV infecting Pole bean and French bean in Bangalore
isolates were determined by analogy with other YMVs infecting legumes
in India as 889 bp and the length of the amino acid sequence is 257.
YMV of pole bean can be managed effectively through an integrated
module viz., border crop (BC) with African tall maize + seed treatment
(ST) with Imidacloprid 70% WS (5.0 gm/kg seed) + use of reflective mulch
(RM) + spraying of Lastraw at 5 ml/ lit at 30 days after sowing (DAS)
(SP1) spraying of Triazophos 40 EC at 0.175% at 45 (SP2) + spraying with
Thiomethoxam 25 WDG at 0.05% at 60 DAS (SP3).
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Keywords
beans, diseases, dna, viruses, wood, kidney beans, biological phenomena, yields, vegetables, planting