MOLECULAR DIAGNOSIS AND CLINICO-THERAPEUTIC STUDIES OF ANAPLASMOSIS IN GOATS OF WAYANAD DISTRICT
| dc.contributor.advisor | P, Vinu David | |
| dc.contributor.author | SHAJU, HONEY | |
| dc.date.accessioned | 2021-01-07T08:37:54Z | |
| dc.date.available | 2021-01-07T08:37:54Z | |
| dc.date.issued | 2019-10-18 | |
| dc.description.abstract | The present study on “Molecular detection and clinico-therapeutic studies of anaplasmosis in goats of Wayanad district” was carried out at the Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Sciences, Pookode during the period from March to December 2018. The aim of the study was to find out the occurrence of anaplasmosis in goats of Wayanad district, molecular detection and differentiation of Anaplasma organisms by PCR, to study the hemato-biochemical alterations associated with caprine anaplasmosis and for evaluating the treatment response in caprine anaplasmosis. Blood samples from 120 goats with clinical signs of anaemia, anorexia and tick infestation were subjected to detailed blood smear examination and PCR. Hematological and serum biochemical parameters were evaluated. Out of the total samples examined, 20 samples which were supposed to be positive by blood smear examination were negative on PCR and other 22 samples which were positive on PCR were negative by blood smear examination. Positive samples were send for sequencing and all the goats were having Anaplasma bovis infection. The main clinical signs exhibited by goats with A.bovis infection were anaemia, anorexia, fever, debility, loss of appetite, jaundice and swollen pre-scapular lymph nodes. The overall incidence of anaplasmosis was 18.33 per cent. Females were commonly affected than males. More number of animals within age group of one to two years were affected with A.bovis. Hematology revealed significant increase in WBC count while hemoglobin, VPRC and MCH values showed significant decrease during the period of infection. Serum biochemistry revealed significant increase in total protein and albumin levels. Positive animals were divided randomly into two treatment groups. Group 1 was treated with oxytetracycline @ 20mg/kg body weight intravenously for three days and Group II with long acting enrofloxacin @ 7.5 mg/kg body weight intramuscularly. Excellent clinical and hematological response were observed in both treatment groups by fourth day. Since the A.bovis organisms detected prior to the treatment disappeared on fourth and fourteenth day on PCR from all animals in both treatment groups, both enrofloxacin or oxytetracycline were equally effective in treating anaplasmosis caused by A. bovis. Polymerase chain reaction was highly sensitive compared to blood smear examination in detecting A.bovis. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810159355 | |
| dc.keywords | 16S rRNA gene ,Anaplasma genus,msp4 gene ,anaplasmosis , A. bovis | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 81 | en_US |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD | en_US |
| dc.research.problem | ANAPLASMOSIS IN GOATS OF WAYANAD DISTRICT | en_US |
| dc.sub | Clinical Veterinary Medicine, Ethics and Jurisprudence | en_US |
| dc.theme | MOLECULAR DIAGNOSIS AND CLINICO-THERAPEUTIC STUDIES OF ANAPLASMOSIS IN GOATS OF WAYANAD DISTRICT | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | MOLECULAR DIAGNOSIS AND CLINICO-THERAPEUTIC STUDIES OF ANAPLASMOSIS IN GOATS OF WAYANAD DISTRICT | en_US |
| dc.type | Thesis | en_US |