IN VITRO EVALUATION OF ANTHELMINTIC PROPERTIES OF THE METHANOLIC AND HYDRO ALCOHOLIC EXTRACTS OF ANNONA MURICATA ON HAEMONCHUS CONTORTUS.
| dc.contributor.advisor | SRINIVASA RAO .K (MAJOR) | |
| dc.contributor.advisor | SREEDEVI .c | |
| dc.contributor.advisor | Ravi Kumar .P | |
| dc.contributor.author | SRI MOUNIKA .G | |
| dc.date.accessioned | 2024-07-06T04:59:34Z | |
| dc.date.available | 2024-07-06T04:59:34Z | |
| dc.date.issued | 2023-06 | |
| dc.description.abstract | Haemonchus contortus is the most pathogenic nematode in sheep and has developed resistance to all commercial anthelmintics throughout the world. In this context, the use of medicinal plants represented as an alternative to chemical anthelmintics. The present study was conducted to evaluate the in vitro anthelmintic effect of crude hydroalcoholic (HE) and methanol extracts (ME) of Annona muricata fruit peel, seed and bark against eggs, infective larvae, and adults of H. contortus by egg hatch assay (EHA), the larval paralysis assay (LPA) and worm motility inhibition assay (WMIA) at different concentrations (0.3 to 81 mg/mL) using albendazole as a reference drug. All extracts exhibited significant (P<0.001) dose and or time dependant anthelmintic effects by inhibiting egg hatching and causing larval paralysis, and mortality of worms compared to negative control, and the effect was comparable with albendazole. At higher (81 mg/mL) concentration, A. muricata extracts showed 96 to 100% efficacy in EHA and 100% efficacy in LPA. In WMIA, worms were completely immobilized in extracts within 1 h post-exposure. Overall performance of the hydroalcoholic extracts of the plant on egg, larva, and adult H. contortus was better than the methanol extract of the same plant except for the seed. At lower (9 and 3 mg/mL) concentrations extracts were effective on egg and larval stage of H. contortus, but did not inhibit the motility of worms. The respective effective dose 50% (ED50) with 95% confidence intervals were estimated. The hydroalcoholic extracts of A. muricata fruit peel (ED50=1.28 mg/mL) and seed (ED50=1.29 mg/mL) significantly inhibited egg hatching, whereas fruit peel extracts significantly inhibited larval motility (ED50=2.71 mg/mL) and adult worm motility (ED50=12.63 mg/mL), indicating their anthelmintic activity. Quantitative and qualitative phytochemical screening of extracts revealed the presence of alkaloids, flavonoids, tannins, amino acids, terpenoids and saponins, which could be accountable for the anthelmintic activity observed. Alkaloids were exclusively present in fruit peel HE. The results confirm the traditional use of A. muricata as a natural anthelmintic, however, further in vivo studies are warranted to validate these effects | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810211517 | |
| dc.language.iso | English | |
| dc.pages | 129 | |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA | |
| dc.sub | Veterinary Parasitology | |
| dc.theme | IN VITRO EVALUATION OF ANTHELMINTIC PROPERTIES OF THE METHANOLIC AND HYDRO ALCOHOLIC EXTRACTS OF ANNONA MURICATA ON HAEMONCHUS CONTORTUS. | |
| dc.these.type | M.V.Sc. | |
| dc.title | IN VITRO EVALUATION OF ANTHELMINTIC PROPERTIES OF THE METHANOLIC AND HYDRO ALCOHOLIC EXTRACTS OF ANNONA MURICATA ON HAEMONCHUS CONTORTUS. | |
| dc.type | Thesis |