Effect of nutri-priming on physiological, biochemical parameters and yield attributes in wheat (Triticum aestivum L.) for mitigation ofterminal heat stress using salts of boron.

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Date
2022, 2022
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Department of Plant Physiology Institute of Agricultural Sciences Banaras Hindu University Varanasi
Department of Plant Physiology Institute of Agricultural Sciences Banaras Hindu University Varanasi
Abstract
The present experiment was carried out in three stages, stage one was named as experiment 1 in which screening was done to determine the ideal concentration of the two boron salts, boric acid and borax for two wheat varieties, HUW-468 (Temperature sensitive, V1) and HUW-510 (Temperature tolerant, V2). Except for germination percentage, which was determined at 24, 48, 72, 96, and 120 hours after germination, data for each morpho-physical and biochemical parameter were recorded at 3, 5, 7, and 9 days of investigation.In experiment I, treatments included non-primed, hydroprimed, 2, 4, 8 10mM boric acid, 0.5, 1.0, 1.5, and 2mM borax salts represented as P1, P2, P3, P4, P5, P6, P7, P8, P9 and P10, respectively. After screening it was found that P5 and P10 showed best result for both the wheat varieties HUW-468 and HUW-510. Stage two and three were named as experiments II and III, which were conducted during Rabi season during 2019-20 and 2020-21, respectively in pots kept inpolyhouse. Two different sowing conditions, i.e., timely and late sown conditions, with two different genotypes of wheat, HUW-510 and HUW-468 were considered for the study.Crop planted too late (IInd fortnight of December)caused heat stress condition for the experiments. The best concentrations of boric acid (P5) and borax (P10) from experiment I were considered for priming in experiment II and III to evaluate the effect of nutrient priming on physiological, biochemical, antioxidants and yield properties of wheat during heat stress condition. For experiment II and III, treatments includedC1; non-primed seed (control), C2; hydro-primed, C3; 8mM boric acid primed and C4; 2mM borax primed ones.At 30, 60, and 90 days after sowing (DAS), observations regarding physiological, biochemicaland antioxidant parameters weredetermined, whereas data regarding developing grains were observed at 70, 80 and 90 DAS, and regarding yield and yield attributes,data were recorded at the time of harvesting. Overall, from all these aforementioned parameters, late sown treatment declined in all the above mentioned parameters, and it was also found that C3 performed better for Variety V1, and C4 showed best result for variety V2; nutri-primed (C3 and C4) sets performed better than the non-primed (C1) and hydroprimed(C2) sets in both timely and late sown conditions.
The present experiment was carried out in three stages, stage one was named as experiment 1 in which screening was done to determine the ideal concentration of the two boron salts, boric acid and borax for two wheat varieties, HUW-468 (Temperature sensitive, V1) and HUW-510 (Temperature tolerant, V2). Except for germination percentage, which was determined at 24, 48, 72, 96, and 120 hours after germination, data for each morpho-physical and biochemical parameter were recorded at 3, 5, 7, and 9 days of investigation.In experiment I, treatments included non-primed, hydroprimed, 2, 4, 8 10mM boric acid, 0.5, 1.0, 1.5, and 2mM borax salts represented as P1, P2, P3, P4, P5, P6, P7, P8, P9 and P10, respectively. After screening it was found that P5 and P10 showed best result for both the wheat varieties HUW-468 and HUW-510. Stage two and three were named as experiments II and III, which were conducted during Rabi season during 2019-20 and 2020-21, respectively in pots kept inpolyhouse. Two different sowing conditions, i.e., timely and late sown conditions, with two different genotypes of wheat, HUW-510 and HUW-468 were considered for the study.Crop planted too late (IInd fortnight of December)caused heat stress condition for the experiments. The best concentrations of boric acid (P5) and borax (P10) from experiment I were considered for priming in experiment II and III to evaluate the effect of nutrient priming on physiological, biochemical, antioxidants and yield properties of wheat during heat stress condition. For experiment II and III, treatments includedC1; non-primed seed (control), C2; hydro-primed, C3; 8mM boric acid primed and C4; 2mM borax primed ones.At 30, 60, and 90 days after sowing (DAS), observations regarding physiological, biochemicaland antioxidant parameters weredetermined, whereas data regarding developing grains were observed at 70, 80 and 90 DAS, and regarding yield and yield attributes,data were recorded at the time of harvesting. Overall, from all these aforementioned parameters, late sown treatment declined in all the above mentioned parameters, and it was also found that C3 performed better for Variety V1, and C4 showed best result for variety V2; nutri-primed (C3 and C4) sets performed better than the non-primed (C1) and hydroprimed(C2) sets in both timely and late sown conditions.
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Seed priming
Seed priming
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