Effect Of Addition Of Glutathione On Tyrosine Phosphorlation And Apoptosis Like Changes In Cryopreserved Hariana Bull Semen

dc.contributor.advisorSingh, Vijay
dc.contributor.authorShah, Nadeem
dc.date.accessioned2017-11-10T06:57:29Z
dc.date.available2017-11-10T06:57:29Z
dc.date.issued2016-07-27
dc.descriptionEffect Of Addition Of Glutathione On Tyrosine Phosphorlation And Apoptosis Like Changes In Cryopreserved Hariana Bull Semenen_US
dc.description.abstractThe present study evaluated the beneficial effects of incorporation of Glutathione as an additive in Tris egg yolk based extender in Hariana bull semen opted for ultralow freezing. The study valuated physical seminal attributes (motility, livability and membrane integrity), process of ryocapacitation, immunoblotting for identification of tyrosine phosphorylated proteins, immunolocalization of osine phosphoproteins in spermatozoa, and apoptosis like changes in terms of mitochondrial transmembrane potential and DNA integrity during after equilibration and thawing. Ten ejaculates from four Hariana bulls were divided into three aliquots: One aliquot diluted with egg yolk tris citrate (EYTC) extender (Control), second aliquot was diluted with EYTC but supplemented with 0.5Mm Glutathione (T1) and the third aliquot was diluted with EYTC and supplemented with 1.0Mm Glutathione (T2) and were cryopreserved. Semen evaluation at equilibration and post-thaw showed supplementation of Glutathione (0.5Mm) to EYTC extender significantly (P < 0.05) increased motility, viability and membrane integrity of spermatozoa. The degree of cryocapacitation was significantly (P < 0.05) decreased in Glutathione supplemented group. Immunoblot revealed six proteins which were tyrosine phosphorylated and protein of 30kDa (p30) showed differential variation in intensity in the three samples. There was significant reduction in band intensity of 30kDa in T1 as compared to control and T2. It was also found that tyrosine phosphorylated proteins were differently located during different stages of semen preservation. Addition of GSH significantly decreased percentage of spermatozoa showing fragmented DNA after thawing as compared to control. Along with this, GSH supplementation significantly increased the percentage of spermatozoa with high transmembrane mitochondrial potential. The result of the present study clearly demonstrated beneficial effects of Glutathione supplementation on post thaw cryocapacitation and apoptosis like changes in spermatozoa and it can be suitably incorporated for long term preservation of spermatozoa.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810035331
dc.keywordsGlutathione, Tyrosine Phosphorlation, Apoptosis, Hariana Bull Semenen_US
dc.language.isoenen_US
dc.pages66, xiien_US
dc.publisherU.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, (DUVASU), Mathura - 281001en_US
dc.research.problem1. To study the cryoprotective effect of addition of glutathione on Tyrosine phosphorylation of cryopreserved Hariana bull semen. 2. To evaluate the effect of glutathione on sperm DNA integrity of cryopreserved Hariana bull semen.en_US
dc.subVeterinary Gynaecology and Obstetricsen_US
dc.subjectnullen_US
dc.themeEffect Of Addition Of Glutathione On Tyrosine Phosphorlation And Apoptosis Like Changes In Cryopreserved Hariana Bull Semenen_US
dc.these.typeM.V.Sc.en_US
dc.titleEffect Of Addition Of Glutathione On Tyrosine Phosphorlation And Apoptosis Like Changes In Cryopreserved Hariana Bull Semenen_US
dc.typeThesisen_US
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