MORPHOLOGICAL CHARACTERIZATION AND PROPGATION STUDIES IN Embelia ribes Burm f. - AN ENDANGERED MEDICINAL PLANT
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Date
2016-07-19
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University of Agricultural & Horticultural Sciences, Shivamogga (COH, MUDIGERE)
Abstract
An experiment was carried out to study the propagation of Embelia ribes Burm f. at the Department of Plantation, Spices, Medicinal and Aromatic crops, College of Horticulture, Mudigere, during the year 2015-16. The work was initiated to standardize the seed and vegetative propagation, tissue culture protocol and morphological characterization of this important RET medicinal plant. Among the different germination inducing treatments, the seeds treated with gibberellic acid at 750 ppm showed higher germination rate (2.07) and seedling vigour (2258.13). Per cent germination (65.33%) was maximum seeds treated with H2SO4 2% + GA3 750 ppm. In vegetative propagation, stem cuttings treated with IBA 2000 ppm recorded maximum sprouting percentage (36.00) and rooting percentage (36.00) at 120 days after planting. For tissue culture, nodal segments were used as explant and among various combinations, satisfactory results were obtained in terms of number of shoots and number of leaves by using a combination of TDZ (2.0mg/l )+ Kin (1.0 mg/l).
To study the morphology, 5 accessions of E. ribes Burm f. maintained at Field Gene Bank, Division of Plant Genetic Resources, Indian Institute of Horticultural Research (IIHR), Bangaluru were studied for 14 quantitative morphological characters. High genetic advance over mean coupled with high heritability was observed in characters like fruit yield per plant (98.50%), leaf width (56.31%), leaf area (10.54%), leaf length (16.47%), fruit diameter (39.44%) and plant height (39.53%). Correlation study revealed that plant height, leaf length, leaf width and fruit length had significant positive correlation with seed yield per plant. In path analysis, leaf length, leaf width, leaf area, fruit diameter and seed length had high positive direct effects on yield per plant.
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