Cloning of Erns Gene of Classical Swine Fever Virus and Characterization of the Expressed Recombinant Protein in Prokaryotic System
Loading...
Date
2013-10
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Vet. J.
Abstract
Classical swine fever virus Erns gene (681 bp) was cloned into pET22b vector using DH5 a cells and further expression was done by transforming into BL21 (DE3) Codon Plus RIL cells. The recombinant protein expression was conīrmed by SDS-PAGE and western blotting methods using both monoclonal and polyclonal antibodies in dimeric form under non-denaturing condition retaining the conformational epitopes. This could be used as a coating antigen for developing discriminatory diagnostic ELISAs for regular sero-monitoring of animals and also during outbreak situations.