IN VITRO PROPAGATION OF A BIOTYPE OF CRAB APPLE [MALUS BACCATA (LINN.) BORKH. (SHILLONG)]
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Date
2019-02
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UHF,NAUNI
Abstract
ABSTRACT
The present studies were made to establish and proliferate shoots using axillary buds
as explants during in vitro propagation of crab apple biotype Shillong. Shoot cuttings were
collected in different months of the year from trees growing at the Dhanda farm of the
Regional Research Station of IARI, Shimla (H.P.). Surface sterilization with 1.0% NaOCl for
20 mins was found more effective as compared to higher concentrations. Rinsing of phenols
in liquid MS medium containing PVP (1g/l) and AC (1g/l) was found essential for explants
survival and successful establishment of buds. The explants of 1-1.5cm size resulted in
medium phenol intensity and maximum bud break (6.67%). MS medium supplemented with
2mg/l BA, 0.5 mg/l GA3 and 0.1 mg/l IBA resulted in maximum bud break of 40.13% and
bud survival of 19.66% after 1st subculture when buds were collected in April month.
However, some of the proliferated shoots did not grow further which may be due to the
internal contamination of bacteria. These shoots turned brown and became necrotic. Shoots
did not induce multiple/axillary shoots in four combinations of growth regulators. Addition of
appropriate antibiotics in culture medium to inhibit the bacterial growth or meristem culture
may be used in fu
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