MAPPING OF GENE(S) FOR RESISTANCE TO POWDERY MILDEW IN SESAMUM (Sesamum indicum L.)

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dc.contributor.advisorANURADHA, G
dc.contributor.authorSRAVANI, DINASARAPU
dc.date.accessioned2016-06-09T12:18:00Z
dc.date.available2016-06-09T12:18:00Z
dc.date.issued2011
dc.description.abstractPowdery mildew is a serious disease of sesamum (Sesamum indicum L). It occurs in epidemics under heavy rainfall conditions followed by low night temperatures and high humid conditions. Powdery mildew causes yield loss of 25 to 50% depending upon the severity. Classical breeding for incorporation of resistance using backcrossing is difficult because consistent disease reaction in the field is hard to achieve due to environmental factors. Identification of molecular markers linked to powdery mildew resistant gene(s) is one approach to improve selection for resistant cultivars. The objective of this study was to identify the mode of inheritance of disease resistance and to use microsatellite (SSR) markers to identify markers linked to resistance gene(s) and map gene(s) resistance to powdery mildew. In the present study Swetha til, a high yielding popular white seeded variety but susceptible to powdery mildew and BB3-8 accession of Sesamum mulayanum resistant to powdery mildew from RARS, Jagtial were selected as parents for hybridization. F1 was developed by crossing the resistant parent ( BB3-8 accession of Sesamum mulayanum ) and the susceptible parent (Swetha til) during Late summer, 2009. F1 was selfed during Kharif 2010 to generate F2. Four populations including P1, P2 , F1, F2 were sown during Late summer, 2010 to screen powdery mildew under field conditions. The F1 of the cross between Swetha til and BB3-8 accession of S.mulayanum exhibited susceptible reaction to the disease suggesting resistance to be governed by recessive gene. On screening of 104 plants of F2 population, the plants segregated into 61 susceptible and 43 resistant plants. Chi-square analysis showed the observed ratio to confirm the expected ratio of 9:7 ratio (susceptible : resistant) and the resistance to disease was governed by two pairs of recessive genes contributed by S.mulayanum. The two parents were screened for parental polymorphism using 300 sesame microsatellite markers, of which 240 showed clear amplification pattern and 24 markers exhibited polymorphism (10%) and the same were used for genotyping. DNA from 104 F2 progenies were collected for genotyping with 24 Polymorphic SSR primers. Molecular linkage map was constructed with SSR markers SM10 178 and SM10 176 using the MAPMAKER/EXP version 3.0 Both the markers were mapped to one and the same linkage group at a distance of 14.2 cM. These two markers were found to be linked to the powdery mildew resistance gene.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/67100
dc.language.isoenen_US
dc.publisherACHARYA N.G. RANGA AGRICULTURAL UNIVERSITYen_US
dc.relation.ispartofseriesD8868;
dc.subGenetics and Plant Breeding
dc.subjectdiseases, genetics, genes, planting, oilseeds, polymorphism, chromosome mapping, dna, breeds (animals), cropsen_US
dc.subjectSesamum indicum Len_US
dc.these.typeM.Sc
dc.titleMAPPING OF GENE(S) FOR RESISTANCE TO POWDERY MILDEW IN SESAMUM (Sesamum indicum L.)en_US
dc.typeThesisen_US
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