Siderophore production by pseudomonas species and its role in disease control of green gram (Vigna radiata) caused by rhizoctonia solani

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dc.contributor.advisorSindhu, S.S.
dc.contributor.authorSahu, Govind Kumar
dc.date.accessioned2016-11-30T08:56:36Z
dc.date.available2016-11-30T08:56:36Z
dc.date.issued2006
dc.description.abstractIn the present study, sixty-five bacterial isolates were obtained from the rhizosphere soil of chickpea and green gram plants by plating serial dilutions on King’s B media. Fifty-eight isolates were identified as Pseudomonas based on colony morphology, pigment characteristics and oxidase test. These fifty-eight Pseudomonas isolates alongwith 22 reference strains were tested for siderophore production using universal chromo-azurol S assay method on MM9 medium and iron-deficient succinate medium. Thirty-seven Pseudomonas strains/isolates were found to produce siderophore on MM9 medium and the diameter of halo zone varied with different Pseudomonas isolates. Production of siderophores was found more on MM9 medium as compared to succinate medium. Bacterial colony growth (G) and halo zone (H) size were measured on MM9 medium and H/G ratios varied from 1.44 to 10.24 in different Pseudomonas isolates. Seventeen Pseudomonas isolates were used for optimization of cultural conditions for enhanced production of siderophores. Replacement of glucose with sucrose, sodium gluconate or sodium malate supported siderophore production whereas arabinose addition repressed siderophore production in most of the Pseudomonas isolates. Increased concentration of ammonium chloride (0.2%) also decreased siderophore production. Addition of Fe-EDTA salt at low 5 M concentration showed less suppressive effect whereas at 10 M concentration more inhibitory effect on siderophore production was observed. Production of siderophore was found more at pH 7.0 in comparison to cultures grown in media with pH 8.0 and pH 6.0. Siderophore producing Pseudomonas isolates inhibited the growth of phytopathogenic fungi Fusarium oxysporum, Rhizoctonia solani and Pythium aphanidermatum. Large inhibition zones of fungal growth were observed on PDA medium in comparison to NA medium plates. Coinoculation of Pseudomonas cultures caused reduction in root rot disease symptoms under pot house conditions and disease control varied from 33.4 to 100 per cent with different Pseudomonas cultures. Coinoculation of Pseudomonas isolates MP20, MPS54, CP56 and CPS67 with Bradyrhizobium strain SMR15 also enhanced the nodule number, nodule fresh weight and plant dry weight as compared to Bradyrhizobium-inoculated or uninoculated control plants at 30, 45 and 60 days of plant growth. Coinoculation of Pseudomonas strain CP56 with Bradyrhizobium strain and R. solani showed maximum increase (275.8%) in plant dry weight at 60 days in comparison to control plants and completely suppressed the root rot disease under pot house conditions. The better performance of siderophore-producing Pseudomonas strains in relation to symbiotic performance and disease control indicated that Pseudomonas strains could be exploited for disease suppression and plant growth promotion under field conditions.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/88281
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subMicrobiologyen_US
dc.these.typeM.Sc
dc.titleSiderophore production by pseudomonas species and its role in disease control of green gram (Vigna radiata) caused by rhizoctonia solanien_US
dc.typeThesisen_US
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