STUDIES ON IN VITRO AND IN PLANTA TRANSFORMATION PIGEONPEA (Cajanus cajan L.) cv. ICPL-8863 (Maruti)
| dc.contributor.advisor | Bhat, Sumangala | |
| dc.contributor.author | Basavanna, S | |
| dc.date.accessioned | 2019-07-26T06:20:14Z | |
| dc.date.available | 2019-07-26T06:20:14Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | In vitro and in planta transformation of pigeonpea was achieved using Agrobacterium tumefaciens strain EHA105 harboring pBinBtS plasmid having crylAc gene linked to the cauliflower mosaic virus (CaMV) 35 S promoter and neomycin phosphotransferase (npt II) gene under the control of nopaline synthase (nos) promoter and terminator. Initially, protocol was developed for in vitro multiple shoot regeneration. Using this protocol in vitro transformation were attempted. Cotyledonary node with cotyledon were pre-cultured on MS medium with 2 mg 1-' BAP for two days, co-cultured for two days in dark, washed with MS broth containing cefatoxime transferred to shoot induction (MS + 2 mg 1' BAP) followed by elongation (MS + 0.1 mg BAP) and rooting medium (MS + 0.5 mg 1-' IBA). Rooted shoots were established in greenhouse. This protocol for pigeonpea transformation using crylAc gene was repeated 8 times through all the steps. In one batch 2 per cent of established plants showed PCR amplification for npt 7/and crylAc primer. In in planta approach, different methods such as simple dipping injury followed by dipping, injection and vaccum infiltration were used at different developmental stages like germination, seedling and at flowering stage. Among different methods vaccum infiltration of the germinating seeds and flower injection were found to be suitable for transformation. | en_US |
| dc.identifier.citation | No. of references126 | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810117033 | |
| dc.keywords | STUDIES ON IN VITRO AND IN PLANTA TRANSFORMATION PIGEONPEA (Cajanus cajan L.) cv. ICPL-8863 (Maruti) | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 138p. | en_US |
| dc.publisher | University of Agricultural Science, Dharwad | en_US |
| dc.research.problem | STUDIES ON IN VITRO AND IN PLANTA TRANSFORMATION PIGEONPEA (Cajanus cajan L.) cv. ICPL-8863 (Maruti) | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | STUDIES ON IN VITRO AND IN PLANTA TRANSFORMATION PIGEONPEA (Cajanus cajan L.) cv. ICPL-8863 (Maruti) | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | STUDIES ON IN VITRO AND IN PLANTA TRANSFORMATION PIGEONPEA (Cajanus cajan L.) cv. ICPL-8863 (Maruti) | en_US |
| dc.type | Thesis | en_US |