Immuno Reactive Recombinant ESAT 6 Like Protein Expressed by ESXO Gene of Mycobacterium avium subsp. paratuberculosis in E.coli
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Date
2018-07
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Association
Abstract
Paratuberculosis caused by Mycobacterium
avium subsp. paratuberculosis is a chronic incurable
infection with worldwide prevalence due to
the limitations in the early diagnosis and lack of
effective vaccine. There is a need to identify the
antigen, which could be candidates for vaccines
with universal ef cacy and for speci c early
diagnosis of paratuberculosis. A novel ESAT gene
family with homology to ESAT6 was identi ed
among mycobacterial species encoding ESAT6
Like proteins. Among the genes of this family
ESXO, was found to be immunodominant and
protects against M. tuberculosis. MAP 4_2856
is an ortholog ESXO gene of M. avium subsp.
paratuberculosis encoding putative ESAT6 like
protein. In this study ESXO gene was cloned in
pET 21b vector and expressed in E.coli. Strong
immuno reactivity with bovine anti M. avium
subsp. paratuberculosis antibodies in immunoblot
analysis supports for an immunodominant
antigen candidate for use.
Description
TNV_IVJ_2018_95(7)45-49
Keywords
Veterinary Science