STUDIES ON MICRO PROPAGATION OF GERBERA (Gerbera jamesonii Bolus) cv. ‘Harley
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Date
2009
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Publisher
UHF,NAUNI,SOLAN
Abstract
ABSTRACT
Studies on micro propagation of Gerbera (Gerbera jamesonii Bolus) cv. ‘Harley’ were carried out in the
tissue culture laboratory of Department of Floriculture and Landscaping of Dr. Y.S. Parmar University of
Horticulture and Forestry, Nauni, Solan (H.P.) during 2008-2009. During the entire course of study, four
experiments were carried out viz., establishment of explants on culture medium, standardization of culture medium
for multiplication, standardization of culture medium for in vitro rooting and standardization of potting mixture for
hardening of plantlets. The surface sterilization of explants with 0.1% HgCl2 for 5 minutes resulted in the highest
uncontaminated growing cultures (70.00%). The earliest culture establishment of capitulum sections (91.74 days)
was recorded with MS medium containing BA (4 mg/l) and IAA (0.5 mg/l). Among explants, capitulum sections
were found to be better multiplier of divisions or clumps (4.89) than the divisions obtained on callus from newly
emerged leaf sections when inoculated on MS medium supplemented with BA (6 mg/l) and Kinetin (2 mg/l).
Maximum number of leaves and leaf length after 8 weeks (11.87 and 4.45 cm, respectively) were obtained in MS
medium supplemented with BA (6 mg/l) and Kinetin (2 mg/l). For in vitro rooting, half strength MS medium
supplemented with 4 mg/l IBA was found to be most effective for per cent rooting, number of roots per clump and
root length whereas earliest rooting was obtained in half strength MS medium supplemented with 5 mg/l NAA. For
hardening of in vitro rooted plantlets, coco peat was found to be a better potting mixture resulting in maximum
survival (90.00%), number of newly emerged leaves (3.10) and leaf length (3.33 cm) after 4 weeks of
transplantation.
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Keywords
sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas