Radiation induced mutation for resistance against dry root rot of chickpea (Cicer arietinum L.)

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dc.contributor.advisorGupta, Om
dc.contributor.authorShivajirao, Pawar Maruti
dc.date.accessioned2017-02-28T05:55:51Z
dc.date.available2017-02-28T05:55:51Z
dc.date.issued2016
dc.description.abstractABSTRACT Chickpea (Cicer arietinum L.) is an important cool-season food legume grown extensively throughout the Indian subcontinent. In India chickpea is being grown in 8.32 million hectare with production of 9.8 million tonnes and 925 - kg/ha productivity (Project coordinators report, 2015-16). Dry root rot of chickpea caused by Rhizoctonia bataticola is becoming serious disease under the changing climate scenario resistant sources in the available genotypes / varieties is the demand of the day. Present investigation entitled “Radiation Induced Mutation for Resistance against Dry Root Rot of Chickpea (Cicer arietinum L.)” was aimed at identification of suitable mutant or a combination of mutants influencing resistance to dry root rot in chickpea. The experimental material was consisted of the population of three selected cultivars of chickpea (JG 63, JG 74, and JG 130) grown in factorial randomized block design in the Seed Breeding Farm, Department of Plant Breeding and Genetics, College of Agriculture, Jabalpur (M.P) under AICRP on chickpea project during Rabi 2014-15 first season 2015-16 Kharif second season offseason nursery Dharwad and Rabi 2015-2016 third season. The laboratory studies were conducted in the Department of Plant Pathology, College of Agriculture, JNKVV, Jabalpur. Dry seeds (10-12% moisture content) of these varieties were irradiated with different doses of gamma rays (150 GY, 200 GY and 400 GY). Another set of pre soaked seeds in distilled water (12hrs.) were treated with ethyl methane sulphonate at different concentration (0.2, 0.3 and 0.4%) prepared for 6 hours with constant intermitted shaking in shaker and washed under running tap water. A portion of seeds irradiated at 150 and 200 GY gamma- ray doses were also treated with 0.3% and 0.4% EMS independently for 6 hours. Present findings revealed that JG 63, JG 74, JG 130 showed significant variation in biological and physiological parameters and disease resistance against dry root rot for mutagenic treatments i.e, 200 GY, 400 GY, 0.3% EMS, 0.4% EMS, 150 GY+0.3% EMS, 200 GY+0.3% EMS. Among 11 treatments, seven have shown effect on biological traits of experimental varieties i.e. change in seedling height; decrease in germination percentage and in plant height as compared to control in M2 and M3 generations. The efficacy of Gamma rays, ethyl methane sulphonate and their combination treatment for resistance in M3 progeny against the dry root rot resistance by using blotter paper technique and sick field screening concluded that 200 GY treatment of physical mutagen, combination treatment of 200 GY+0.2% EMS and in chemical treatment 0.3% EMS shows dry root rot resistance against JG 63 and highly susceptible against two varieties JG 74 and JG130 in M3 generation. JG 63 possess maximum relative water content percent, maximum chlorophyll content and minimum leakage of ions in membrane stability index percent and less percent mortality due to dry root rot and considered as resistant as compared to other varieties JG 74 and JG 130 at different mutagenic treatments in M3 generation.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810002874
dc.keywordsPLANT PATHOLOGYen_US
dc.language.isoenen_US
dc.pages104en_US
dc.publisherJNKVVen_US
dc.subPlant Pathologyen_US
dc.subjectPLANT PATHOLOGYen_US
dc.themePLANT PATHOLOGYen_US
dc.these.typePh.Den_US
dc.titleRadiation induced mutation for resistance against dry root rot of chickpea (Cicer arietinum L.)en_US
dc.typeThesisen_US
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