Detection of Multidrug Resistance MDR Staphylococcus Aureus from Mastitis Infected Cows

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dc.contributor.advisorMhase, P. P.
dc.contributor.authorRamteke Manali Diwakar
dc.date.accessioned2023-11-16T12:12:48Z
dc.date.available2023-11-16T12:12:48Z
dc.date.issued2023-02-20
dc.description.abstractCattle are frequently affected by mastitis, an infection of the mammary gland that lowers milk production and ultimately has an effect on the economy. One of the most common bacteria that cause mastitis is Staphylococcus aureus. S. aureus occurring in mastitis frequently exhibits resistance to commonly employed antibiotics which is of great concern. Therefore, current investigation was planned for studying mastitis in dairy cows, characterization of the major pathogen S. aureus, and its present scenario, especially regarding the antibiotic sensitivity pattern in the area around Shirwal, Satara district in western Maharashtra. For this study a total of 250 Holstein Friesian (HF) crossbred cows from unorganized dairy farms were screened for mastitis. Out of these 68% of cows tested positive for mastitis. Mastitic milk samples were processed for the isolation of pathogenic microorganisms and their antibiotic sensitivity pattern. S. aureus strains were targeted for characterization with phenotypic as well as genotypic methods using 16s rRNA gene and species-specific nuc gene analysis. S. aureus strains were also presented for Antibiotic Susceptibility Test (ABST), and resistant strains showing multidrug resistance (MDR) were subcultured. S. aureus represented the highest resistance to ß lactam group of antibiotics (98.05%) followed by macrolide group (95%). Amongst the drugresistance genes studies, highest number of S. aureus carried ermA (78.33%) gene followed by tetK (61.67%) gene. An attempt was made to synthesize and employ the Green Silver Nanoparticles (GAgNPs) as an alternative therapeutic agent. Different concentrations of an aqueous leaf extract of Azadirachta indica were used successfully as a reducing agent for the biosynthesis of GAgNP from Silver Nitrate producing brown colored suspension in 48 hours. GAgNPs were characterized by Spectrophotometry where peak absorbance was produced at 405 nm. Transmission Electron Microscopy (TEM) of GAgNPs revealed spherical nanoparticles of 10 to 70 nm in size which readily attached to the bacterial cell membrane, disrupting it and demonstrating bactericidal activity.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810201255
dc.keywordsVeterinary Microbiology
dc.keywordsMultidrug Resistance
dc.keywordsStaphylococcus
dc.keywordsAureus
dc.keywordsMastitis
dc.keywordsInfected Cow
dc.language.isoEnglish
dc.pages167
dc.publisherMAFSU, NAGPUR
dc.subVeterinary Microbiology
dc.themeMultidrug Resistance MDR Staphylococcus Aureus from Mastitis Infected Cows
dc.these.typeM.V.Sc.
dc.titleDetection of Multidrug Resistance MDR Staphylococcus Aureus from Mastitis Infected Cows
dc.typeThesis
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