Detection of Multidrug Resistance MDR Staphylococcus Aureus from Mastitis Infected Cows
Loading...
Files
Date
2023-02-20
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MAFSU, NAGPUR
Abstract
Cattle are frequently affected by mastitis, an infection of the mammary gland
that lowers milk production and ultimately has an effect on the economy. One of the
most common bacteria that cause mastitis is Staphylococcus aureus. S. aureus
occurring in mastitis frequently exhibits resistance to commonly employed antibiotics which is of great concern. Therefore, current investigation was planned for studying
mastitis in dairy cows, characterization of the major pathogen S. aureus, and its
present scenario, especially regarding the antibiotic sensitivity pattern in the area
around Shirwal, Satara district in western Maharashtra. For this study a total of 250
Holstein Friesian (HF) crossbred cows from unorganized dairy farms were screened
for mastitis. Out of these 68% of cows tested positive for mastitis. Mastitic milk
samples were processed for the isolation of pathogenic microorganisms and their
antibiotic sensitivity pattern. S. aureus strains were targeted for characterization with
phenotypic as well as genotypic methods using 16s rRNA gene and species-specific
nuc gene analysis. S. aureus strains were also presented for Antibiotic Susceptibility
Test (ABST), and resistant strains showing multidrug resistance (MDR) were
subcultured. S. aureus represented the highest resistance to ß lactam group of
antibiotics (98.05%) followed by macrolide group (95%). Amongst the drugresistance
genes studies, highest number of S. aureus carried ermA (78.33%) gene
followed by tetK (61.67%) gene.
An attempt was made to synthesize and employ the Green Silver
Nanoparticles (GAgNPs) as an alternative therapeutic agent. Different concentrations
of an aqueous leaf extract of Azadirachta indica were used successfully as a reducing
agent for the biosynthesis of GAgNP from Silver Nitrate producing brown colored
suspension in 48 hours. GAgNPs were characterized by Spectrophotometry where
peak absorbance was produced at 405 nm. Transmission Electron Microscopy (TEM)
of GAgNPs revealed spherical nanoparticles of 10 to 70 nm in size which readily
attached to the bacterial cell membrane, disrupting it and demonstrating bactericidal
activity.