Molecular characterization of Gluconacetobacter diazotrophicus isolated from different field crops and its interactive effect with microbial consortia on growth and yield of Maize (Zea mays L.) and Finger millet (Eleusine coracana L.)
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Date
2017-12-02
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
The Gluconacetobacter species are known to fix large amount of nitrogen without
the formation of nodules in monocot plants particularly in sugarcane. This led to
recognition of its importance in agriculture. In the present study, four
Gluconacetobacters were isolated from Finger millet (R-01), Maize (MZ-01), Sorghum
(SO-01) and Sugarcane (SU-01). The four isolates produced brown pigments on PDA
and GYC media and orange pigment on LGI-P medium. Besides, they also produced
IAA, solubilized P and Zn. These isolates were identified as Gluconacetobacter
diazotrophicus by 16S rRNA gene sequence and screened for their efficiency on
increasing growth and yield of maize and finger millet under glass house conditions. The
isolate of maize (MZ-01) and its consortium consisting of G. diazotrophicus MZ-01 + B.
megaterium + G. fasciculatum was found more efficient in enhancing the growth and
yield. Therefore, the G. diazotrophicus MZ-01 and its consortia with 50 % recommended
dose of fertilizers were selected for field studies. In the field experiment also the
consortium of G. diazotrophicus MZ-01 + B. megaterium + G. fasciculatum + 50 % RDF
NP found on par with 100 % RDF NP. Further, microbial population including beneficial
microbes increased as the age of the plants increased and found maximum at flowering
stage in both pot as well as field experiments. Thus, the G. diazotrophicus MZ-01 found
superior bacterial inoculant for field crops and could save 50 % nitrogen and phosphorus
fertilizers.
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