STUDIES ON COST EFFECTIVE MICROPROPAGATION OF GISELA-5 (PRUNUS CERASUS × PRUNUS CANESCENS)CHERRY ROOTSTOCK
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Date
2018-09
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UHF NAUNI
Abstract
ABSTRACT
In the present investigations, a technique for cost effective in vitro propagation of Gisela-5 (Prunus
cerasus x Prunus canescens)- cherry rootstock has been developed. It was observed that table sugar at 40 g/l,
was next best carbon source after sucrose and corn starch at 40 g/l, isabgol at 20 g/l and tapioca seeds at 100 g/l
were found to be the best among the different gelling agents tried. Combination of sucrose + corn starch and
table sugar + corn starch with multiplication rate of 1:5 with average shoot length of 2.30 cm, and proved to be
superior to the nutrient medium containing sucrose + agar (control). Subculturing on the respective medium
gave similar results of multiplication rate and average shoot length. Best rooting of 100 per cent was observed
in one step procedure on half strength MS liquid control, solid control and medium with sucrose + corn starch
(LCR1) whereas, in two step procedure of rooting, maximum rooting (100%) was observed after 48 hours of
dark incubation in half strength MS broth fortified with 0.5 mg/l IBA followed by transfer to half strength MS
basal control. Rooted plantlets were transplanted in sterilized potting mixtures for hardening and kept in the
glasshouse. 60 per cent survival was observed after 4 weeks of transfer in cocopeat when sprayed with 3 %
jeevamrut at regular intervals of 7 days. When the soil was drenched with 20 ml of jeevamrut (3 %), 60 %
survival rate was observed after 4 weeks of transfer. Relative cost of components was analyzed and it was found
that there was a significant cost difference between the control medium and low cost medium. When per plantlet
cost was evaluated, control medium was found to be the most expensive resulting in cost of Rs. 2.16 per plantlet
and the cheapest medium was the one containing corn starch and sucrose yielding the cost of Rs. 0.82 per
plantlet whereas, the medium containing table sugar and corn starch yielded the per plantlet cost of Rs. 0.84.
This proves that corn starch could be used as best low cost gelling agent as an alternate to agar and table sugar
could be used as an cheaper alternate to the expensive sucrose.
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