CULTURABLE MICROBIAL DIVERSITY ASSOCIATED WITH GUT OF APIS SPP. (HYMENOPTERA: APIDAE)
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Date
2015-07-10
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Honeybees are known to harbour microorganisms in the gut which play an important role in their nutrition. In the present study bacterial strains were isolated from foregut, midgut and hindgut of Apis cerana, A. mellifera, A. jlorea and A. dorsata workers and drones and from different gut regions of queen of A. cerana and A. jlorae collected from different regions of Bengaluru. Identification and characterization of microbes was done by gram staining technique for each isolated strains to differentiate between gram positive and gram negative organisms. The morphological characters of all isolated bacterial colonies were also studied. Total bacterial genomic DNA was extracted from the gut and amplified using PCR with 16S rRNA primers. The partial 16S rDNA sequences from Apis spp. revealed the presence of novel bacterial flora, Bacillus spp. which were more predominant in different guts of worker, queen and drone of Apis spp. The gut microflora varied from gut to gut within the caste and also varied from caste to caste within the species. Acinetobacter iwoffi and Klebsiella varricolawas identified only in Apis jlorea drones compared with other castes and species. In Apis dorsata, Pseudomonas sp. was found which was not found in any other species. The phylogenetic analysis of the Apzs spp. using bacterial strains of different gut regions of different castes clearly indicated the microbial diversity which can be used for management of bee health and as antimicrobial agents.
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