High temperature induced changes in oxidative stress, antioxidant system and polypeptide pattern in Indian mustard [Brassica juncea (L.) Czern & Coss.]

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dc.contributor.advisorDhawan, Kamal
dc.contributor.authorBabita Rani
dc.date.accessioned2016-11-16T09:08:08Z
dc.date.available2016-11-16T09:08:08Z
dc.date.issued2009
dc.description.abstractThe present investigations were undertaken to study the changes in lipid peroxidation, antioxidative enzymes, metabolites and protein pattern in Brassica juncea seedling after subjecting to high temperature stress (45±0.5C) and on revival. Two tolerant genotypes viz. BPR-542-6 and NRCDR-02 and two susceptible genotypes viz. NPJ-119 and RGN-152 were screened at 45±0.5C on the basis of time taken to 50% seedling mortality. Lipoxygenase (LOX), malondialdehyde (MDA) and hydrogen peroxide (H2O2) which are indicators of cell membrane damage increased under heat stress in all the genotypes but increase was higher in susceptible genotypes. On recovery, LOX, MDA and H2O2 content decreased in all the genotypes. The activities of antioxidative enzymes viz . superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) increased under high temperature stress . SOD and CAT started decreasing but activity of POX and GR still continued increasing in all the genotypes. But APX enzyme exhibited differential behaviour on revival which increased in tolerant genotypes but decreased in susceptible genotypes. Ascorbic acid and carotenoids increased under heat stress but during recovery, ascorbic acid continued increasing while carotenoids started decreasing in all the genotypes. A major band of 53.12 kDa and minor bands of 100 kDa, 89.12 kDa, 74.13 kDa, 46.76 kDa and 38.9 kDa in tolerant genotypes and protein bands of molecular weight 25.79 kDa and 30.7 kDa in susceptible genotypes appeared under high temperature stress which disappeared when the stress was relieved.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/85755
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiochemistry
dc.subjectSowing, Storage, Physical control, Packaging, Polymers, Germinability, Seed treatment, Storage structures, Hybrids, Maizeen_US
dc.these.typeM.Sc
dc.titleHigh temperature induced changes in oxidative stress, antioxidant system and polypeptide pattern in Indian mustard [Brassica juncea (L.) Czern & Coss.]en_US
dc.typeThesisen_US
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