High temperature induced changes in oxidative stress, antioxidant system and polypeptide pattern in Indian mustard [Brassica juncea (L.) Czern & Coss.]
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Date
2009
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Publisher
CCSHAU
Abstract
The present investigations were undertaken to study the
changes in lipid peroxidation, antioxidative enzymes, metabolites and
protein pattern in Brassica juncea seedling after subjecting to high
temperature stress (45±0.5C) and on revival. Two tolerant genotypes
viz. BPR-542-6 and NRCDR-02 and two susceptible genotypes viz.
NPJ-119 and RGN-152 were screened at 45±0.5C on the basis of
time taken to 50% seedling mortality. Lipoxygenase (LOX),
malondialdehyde (MDA) and hydrogen peroxide (H2O2) which are
indicators of cell membrane damage increased under heat stress in
all the genotypes but increase was higher in susceptible genotypes.
On recovery, LOX, MDA and H2O2 content decreased in all the
genotypes.
The activities of antioxidative enzymes viz . superoxide
dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate
peroxidase (APX) and glutathione reductase (GR) increased under
high temperature stress . SOD and CAT started decreasing but
activity of POX and GR still continued increasing in all the
genotypes. But APX enzyme exhibited differential behaviour on
revival which increased in tolerant genotypes but decreased in
susceptible genotypes. Ascorbic acid and carotenoids increased
under heat stress but during recovery, ascorbic acid continued
increasing while carotenoids started decreasing in all the genotypes.
A major band of 53.12 kDa and minor bands of 100 kDa,
89.12 kDa, 74.13 kDa, 46.76 kDa and 38.9 kDa in tolerant
genotypes and protein bands of molecular weight 25.79 kDa and
30.7 kDa in susceptible genotypes appeared under high temperature
stress which disappeared when the stress was relieved.
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Keywords
Sowing, Storage, Physical control, Packaging, Polymers, Germinability, Seed treatment, Storage structures, Hybrids, Maize