Immunity related gene expression during progression and regression of canine transmissible venereal tumour
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Date
2017-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was conducted on clinical cases of twenty four dogs of either sex or age affected with naturally occurring canine transmissible venereal tumour (CTVT) to evaluate the gene expression during progression and regression of tumour and to evaluate the T cell response toward mitogen Concanavalin A. Animals were randomly divided into four groups consisting of 6 dogs each. Group 1 consists of TVT affected dogs in which vincristine sulphate were not administered. Group-2 consists of normal dogs in which vincristine sulphate were administered. Group-3 consists of TVT affected dogs in which vincristine sulphate were administered. Control group consists of normal dogs in which vincristine sulphate were not administered. The assessment were made on the basis of clinical examination, gross regression after chemotherapy, haemato-biochemical, lymphocyte proliferation assay and expression of gene were studied before and after administration of vincristine sulphate in CTVT affected dogs. On the basis of these parameters, it is concluded that transforming growth factor beta 1 (TGF-β1) was secreted by CTVT cells during progression phase which suppressed the lymphokine activated killer (LAK) cytotoxicity, which is suppressed by CTVT cells secreted IL-6 during the regression phase of the tumour. T- lymphocytes are also associated with the regression phase of CTVT suggested that tumour immunity also plays a major role in tumour regression.
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