Production of Cellulase from Bacillus subtilis under Solid- State Fermentation Using Fiber Wastes of Palmyra Palm

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dc.contributor.authorRoopa, R
dc.contributor.authorCharulatha, M
dc.contributor.authorMeignanalakshmi, S
dc.contributor.authorTANUVAS
dc.date.accessioned2019-01-04T09:20:41Z
dc.date.available2019-01-04T09:20:41Z
dc.date.issued2017-06
dc.descriptionTNV_Ijcmas_2017_6(6)2225-2231en_US
dc.description.abstractIn the present study, the production of cellulase has been carried out using novel natural waste as substrates by Bacillus subtilis isolated from slaughter house waste rumen fluid. Bacillus subtilis was screened for cellulase production by Congo red assay. pH and temperature were optimized. Agricultural wastes such as Hay, fiber waste of palmyra palm and banana bracts were used as a substrate for cellulase production. Substrates with or without pretreatment using NaOH solution were used for solid state fermentation. After 48hrs of fermentation, extracts were drawn and tested for enzyme activity by DNSA method and total protein content by Lowry’s method. Cellulase activity was found to be 0.1519, 0.0759 and 0.05557 μmol ml-1min-1 when fibers of palmyra palm, hay and banana bracts were used as substrates. Raw cellulose content was found to be 37%, 32% and 23.2% in fibers of palmyra palm, hay and banana bracts respectively. Fibers of palmyra palm were found to be having maximum enzyme activity of 0.1519μmol ml-1min-1 at pH 7, at 48hrs of incubation.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810088936
dc.keywordsCellulase, Bacillus subtilis, Palmyra palm, Solid state fermentation, Agricultural waste.en_US
dc.language.isoenen_US
dc.pages2225-2231en_US
dc.publisherExcellent Publishersen_US
dc.relation.ispartofseries;6
dc.subjectVeterinary Scienceen_US
dc.titleProduction of Cellulase from Bacillus subtilis under Solid- State Fermentation Using Fiber Wastes of Palmyra Palmen_US
dc.title.alternativeInternational Journal of Current Microbiology and Applied Sciencesen_US
dc.typeArticleen_US
dc.volume6en_US
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