Phenotypic and molecular characterisation of backcross generations for salt tolerance in wheat (Triticum aestivum L. em.Thell)
| dc.contributor.advisor | Vikram Singh | |
| dc.contributor.author | Sharma, Sudhir | |
| dc.date.accessioned | 2017-11-15T04:23:13Z | |
| dc.date.available | 2017-11-15T04:23:13Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Soil salinity is a major abiotic stress having adverse effects on all growth stages of wheat. In present study, HD 2967 x Kharchia 65 derived F3, BC1F2 (initial salt stress) and BC2F1 (under and field conditions) generations were evaluated for various morpho-physiological traits. A total of 430 seeds out of 750 BC1F2 seeds (57.33%) and 456 out of 700 F3 seeds (65.14%) germinated under salt stress. In all the three generations, ample variation was observed for all traits under study. Biological yield per plant, number of effective tillers per plant, spike length and plant height showed positively significant correlation with grain yield per plant across the generations. Path analysis of all three generations revealed that biological yield and harvest index had high direct effect on grain yield per plant while other traits viz. number of effective tillers per plant, plant height and spike length had significant indirect effect on grain yield per plant through biological yield per plant and harvest index. Marker assisted selection was done for Nax loci; responsible for salt tolerance in wheat. Thirty-seven plants, comprising of 29 BC1F2, 2 F3 and 6 BC2F1 showed amplification for both Nax1 and Nax2 locus. SSR (33) were used to generate allelic profile of selected plants. Molecular analysis showed that average recurrent parent recovery in BC1F2, BC2F1 and F3 plants was 83.18, 81.82 and 86.24%, respectively. Cluster tree analysis showed that HD 2967 and Kharchia 65 were clustered separately and plants in all three generations were clustered near recurrent parent HD 2967. Nine plants selected on the basis of high yield and the presence of Nax loci viz. plant number 50, 11 and 39 of F3, 16, 68 and 95 of BC1F2, and 21, 16 and 11 of BC2F1were advanced in the next generation and will be further backcrossed with the recurrent parent HD 2967 to develop salt tolerant wheat lines. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810035683 | |
| dc.keywords | Nax1, Nax2, Molecular diversity, Salt stress, Wheat | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | Phenotypic and molecular characterisation of backcross generations for salt tolerance in wheat (Triticum aestivum L. em.Thell) | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Phenotypic and molecular characterisation of backcross generations for salt tolerance in wheat (Triticum aestivum L. em.Thell) | en_US |
| dc.type | Thesis | en_US |