Molecular characterization of Eleusine coracana (L.) Gaertn. genotypes for variability in zinc content and development of transgenics with high grain zinc
| dc.contributor.advisor | SHANKAR, A. G. | |
| dc.contributor.author | YAMUNA RANI, B. R. | |
| dc.date.accessioned | 2017-02-23T09:44:19Z | |
| dc.date.available | 2017-02-23T09:44:19Z | |
| dc.date.issued | 2009-10-19 | |
| dc.description.abstract | Zinc deficiency ranks fifth among the most important health risk factors throughout the world. Zn plays a crucial role in structure and function of various proteins, including enzymes, transcription factors, hormonal receptor sites and biological membranes of all organisms. Because Zn plays multiple roles in plant biochemical and physiological processes, even slight deficiencies will cause a decrease in growth, yield and zinc content of edible parts. Therefore, it is necessary to understand the physiological and molecular details of how plants take up, translocate and store zinc. The inadequate amount of Zn in plants has lead to serious health concern to humans. In this regard any approach to improve the Zn content in edible parts is a need of hour. Biofortification, a new approach that relies on conventional plant breeding and modern biotechnology to increase the micronutrient density of staple crops. In the present study we assessed the grain Zn content in different finger millet genotypes and studied the distribution of Zn in different plant tissues and analysed the expression of Zn transporter genes in contrasting genotypes of finger millet grown in presence of various levels of Zn. The results revealed that the contrasting genotypes exhibit differential response in Zn uptake and translocation to shoots from roots subjected to external Zn application. Similarly ZIP family transporters of Zn are differentially expressed in leaves and roots of finger millet under different level of Zn treatments, however, ZIP1 transcript level is high under Zn deficient conditions. The role of AtZIP3 transporter gene was studied in Arabidopsis mutants and showed decreased Zn content in roots suggesting the AtZIP3 was involved in uptake rather than translocation to shoots. Transgenic plants of tobacco and finger millet expressing OsZIP1 were also developed. The transgenic finger millet plants showed better assimilation and gas exchange parameters than wild type plants. Transgenic expressing OsZIP1 under the control of endosperm specific promoter (Bx17) showed higher levels of zinc in grains compared to OsZIP1 plants under constitutive (CaMV35S) promoter, therefore tissue specific expression is a promising approach. | en_US |
| dc.identifier.other | Th-9453 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810002395 | |
| dc.language.iso | en | en_US |
| dc.pages | 189 | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Crop Physiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Genotypes for variability in zinc | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Molecular characterization of Eleusine coracana (L.) Gaertn. genotypes for variability in zinc content and development of transgenics with high grain zinc | en_US |
| dc.type | Thesis | en_US |