Effect of addition of lochia on some characteristics of crossbred bulls’ semen
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Date
2009-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The study was conducted on lochia (postpartum uterine secretion) samples collected from ten crossbred cows and semen from two crossbred bulls. The objective was to standardize the technique of lochia collection from crossbred cows and to study the effect of addition of lochia on progressive sperm motility percentage, live sperm percentage and abnormal sperm percentage of crossbred bulls’ semen. Lochia collection from uterus was done by using Foley’s catheter with syringe which helped to collect varying amount of lochia aseptically. The lochia added semen samples were divided into three treatment groups (T1, T2 and T3) composing different (0.5 ml, 1 ml and 2ml, respectively) volumes of semen and 0.1 ml of lochia. In Control group 0.1 ml normal saline was added instead of lochia in semen sample. The values of overall mean of progressive sperm motility1, Live2 and abnormal3 sperm count in T1, T2, T3 and control group were (64.9 ± 1.7, 36.0 ± 0.6 and 13.6 ± 0.9)1, (64.6 ± 2.0, 66.1 ± 1.8, 65.0 ±2.2, 66.3 ±1.8)2 and (4.9 ± 0.7, 4.8 ± 0.8, 3.9 ± 0.7 and 4.4 ± 0.6)3 at 0 hour, (36.0 ± 0.63, 36.6 ± 1.18, 35.2 ± 1.25, and 37.1 ± 1.32)1, (43.2 ± 2.0, 42.1 ± 1.7, 43.1 ± 2.2 and 42.7 ± 1.8 )2 and (5.5 ± 0.6, 5.3 ± 0.6, 4.9 ± 0.9 and 4.4 ± 1.0)3 after 4 hours and (13.6 ± 0.97, 12.7 ± 1.20, 14.3 ± 1.19 and 14.57 ± 1.35)1, (25.3 ± 1.6, 24.1 ± 1.1, 26.0 ± 1.3 and 24.2 ± 1.3)2 and (5.5 ± 0.6, 5.3 ± 0.6, 4.9 ± 0.9 and 4.4 ± 1.0)3 after 8 hours, respectively. There were no significant differences among these values of control and treatments. The results show that the lochia is not detrimental to the survivability of the sperm. Further detailed investigation with more purified lochia at other dilution rates is needed.