TECHNOLOGY REFINEMENT FOR MICROPROPAGATED Aloe vera L. AND ASSESSMENT OF GENETIC FIDELITY
| dc.contributor.advisor | KANWAR, KAMLESH | |
| dc.contributor.author | SHARMA, SWATI | |
| dc.date.accessioned | 2016-12-28T10:57:01Z | |
| dc.date.available | 2016-12-28T10:57:01Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | ABSTRACT The present investigation aims at “Technology refinement for micropropagated Aloe vera L. and assessment of genetic fidelity”. Surface sterilized shoot buds cultured on control (MS + 3% sucrose + 0.8% agar-agar ) and low cost medium (MS + 3% table sugar + 10% tapioca pearls) supplemented with 2.0 mg/l, 0.5 mg/l and 0.2 mg/l NAA which showed 81.66% and 79.33% establishment respectively. Both control as well as low cost medium were found at par with each other having 81.99% and 81.80% shoot regeneration respectively. 77.67% rooting and 4.33 average roots per shoot were found on 1/4 MS + 3% sucrose + 0.8% agar-agar and 78.00% rooting and 3.67 average roots per shoot on 1/4 MS + 10% tapioca pearls + 3% table sugar. The study has resulted in the reduction cost of the medium by substituting agar-agar and sucrose with tapioca pearls and table sugar. Further, morphological parameters showed that there is similarity between in vitro raised plants. During the RAPDs studies, 17 random decamer primers were used. Total 55 scorable bands were obtained out of which 52 bands found monomorphic and 3 were polymorphic in nature. The similarity coefficient value ranged from 0.94 to 1.00. Similarity of 98% from dendrogram has been observed which was constructed on similarity matrix. | en_US |
| dc.identifier.other | 47885 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/93412 | |
| dc.language.iso | en | en_US |
| dc.sub | Biotechnology | en_US |
| dc.these.type | M.Sc | |
| dc.title | TECHNOLOGY REFINEMENT FOR MICROPROPAGATED Aloe vera L. AND ASSESSMENT OF GENETIC FIDELITY | en_US |
| dc.type | Thesis | en_US |