STUDIES ON PREVALENCE, CHARACTERIZATION AND EFFECT OF ANTIMICROBIAL AGENTS ON Escherichia coli ISOLATES FROM CHICKEN
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Date
2013-01-14
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CSKHPKV, Palampur
Abstract
In the present study, microbiological quality of chicken meat, eggs and their products was
assessed by employing standards plate count and coliform count with special emphasis on
Escherichia coli which is a food borne pathogen of public health importance. A total of
250 samples of raw and ready to eat chicken meat and eggs were screened for the presence
of E. coli. Based on serotyping maximum prevalence was found in raw chicken (13.33%),
followed by shell eggs (8 %), egg products (6.67%) and chicken products (4.76%). Among
the twenty two isolates confirmed by serotyping, 12 isolates belonged to 10 different ‘O’
serogroups viz. O2, O8, O11, O13, O17, O21, O23, O35, O66 and O155 while 5 were
rough strains and 5 were untypable strains. All the isolates were characterized in terms of
antibiotic resistance/sensitivity, haemolysin production, plasmid profiles and presence of
virulent genes. On the basis of PCR, ten out of 22 serotyped isolates revealed the presence
of virulence genes with nine isolates showing the presence of eae and bfpA genes and one
isolate having bfpA and stx1/stx2 genes. Antibiogram studies of the isolates revealed that
59.09% of the isolates were multidrug resistant with maximum isolates showing resistance
to co-trimoxazole (86.36%), followed by tetracycline (68.18%), ofloxacin (40.91%) and
piperacillin/tazobactam (40.91%) while cent per cent sensitivity was observed against
kanamycin. Plasmid profiling revealed multiplicity and random distribution of plasmid
DNA with plasmid bands ranging from 1.2 kb to >10 kb. None of the isolates displayed
hemolytic activity. Due to ever increasing problem of antibiotic resistance, effect of both
methanolic and aqueous extracts of pomegranate peels, orange peels, curry leaves, radish
leaves, seabuckthorn leaves and ginger rhizomes was evaluated against E. coli. Only the
aqueous and methanolic extracts of pomegranate peels were found effective against E. coli
at the concentration of 8%, 9% and 10%.
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