Effects of Testosterone, Estrogen and Progesterone on TNF-α Mediated Cellular Damage in Rat Arthritic Synovial Fibroblasts

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dc.contributor.authorGanesan, K.
dc.contributor.authorBalachandran, C.
dc.contributor.authorManohar, B. Murali
dc.contributor.authorPuvanakrishnan, R.
dc.contributor.authorTANUVAS
dc.date.accessioned2016-08-16T11:17:23Z
dc.date.available2016-08-16T11:17:23Z
dc.date.issued2012
dc.description.abstractSexual dimorphism is a well-established phenomenon in rheumatoid arthritis, with women exhibiting higher disease severity. Understanding the role of sex hormones using in vivo animal models is limited due to the systemic effects as well as the difficulty in exploring different dose combinations of the hormones simultaneously. However, cell culture systems pose ideal systems for exploring different combinations and concentrations of the hormones simultaneously. In this study, the procedure for isolation of arthritic fibroblasts was standardized using a combination of collagenase and trypsin based on maximal yield and viability after employing different enzymatic disaggregation procedures. The cultures synovial fibroblasts from arthritic rats did not differ significantly from normal rat firbroblasts in terms of proliferation or secretion of inflammatory mediators. Stimulation of fibroblasts with TNF-α was standardized and TNF-α stimulated rat arthritic synovial fibroblasts exhibited an ideal in vitro system for screening antiinflammatory molecules. The effects of physiological and pharmacological concentrations of testosterone, estrogen and progesterone were studied on TNF-α induced cellular damage in rat arthritic synovial fibroblasts. the results showed that estrogen and testosterone exerted antiinflammatory effects on rat arthritic synovial fibroblasts at physiological and pharmacological concentrations. However, there was no significant difference in the effects between physiological and pharmacological concentrations. Progesterone independently did not show any protective effects. In combination with physiological concentrations of estrogen, progesterone abrogated estrogen's protective effect but it exhibited protection in combination with pharmacological concentrations of estrogen.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/72515
dc.language.isoenen_US
dc.publisherRheumatol Int.en_US
dc.subjectArthritisen_US
dc.subjectSteroidsen_US
dc.subjectSynovial Fibroblastsen_US
dc.subjectTNFen_US
dc.subjectCytokinesen_US
dc.titleEffects of Testosterone, Estrogen and Progesterone on TNF-α Mediated Cellular Damage in Rat Arthritic Synovial Fibroblastsen_US
dc.typeArticleen_US
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