POLYMORPHISM OF IFNA16 AND CAST GENES IN SWINE POPULATION OF ANDHRA PRADESH
Loading...
Files
Date
2024-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA
Abstract
A total of 218 pigs namely LWY and SVVU T-17 were randomly selected and
blood samples were collected to determine genetic polymorphism of CAST and
IFNA16 genes by using PCR-RFLP method.
The genomic DNA was amplified by PCR and product size of 376bp for CAST
and 237bp for IFNA16 gene was obtained. The PCR products of CAST and IFNA16
genes were digested with HinfI restriction enzyme which recognizes the SNP 76872
G>A and 413G>A respectively and incubated at 37o overnight. The restriction
fragment sizes of 376, 247, 129 bp (3 bands), 247, 129(2 bands) and uncut fragment of
376bp were observed for CAST/HinfI locus, whereas IFNA16/HinfI (rs701706389)
genotyping revealed that two genetic groups were monomorphic with A allele. Three
different types genotypes were identified (AA, GA and GG) based on the different
patterns and two alleles namely A and G. For CAST gene the genotypic and allelic
frequencies of AA, GA, GG and A and G were found to be 0.39, 0.49, 0.12 and 0.63,
0.37 (LWY); 0.008, 0.47, 0.522 and 0.24, 0.76 (SVVU T-17). The Chi-Square value
for CAST locus revealed significant difference (P<0.01) in SVVU T-17, whereas it was
non-significant for LWY. Chi-Square differences in genotypic and allelic frequencies
at CAST/HinfI locus between LWY and SVVU T-17 shown significant differences
(P<0.01) between them. The observed heterozygosity (Ho) values ranged from 0.47 to
0.49 and the expected heterozygosity (He) values ranged from 0.37 to 0.46 for CAST
gene. The Polymorphic Informative Content (PIC) value was medium and ranged from
0.29 to 0.36 for CAST gene. The Fixation index (FIS) value were found to be negative
for two genetic groups and ranged from -0.27 to -0.06.
The results revealed the existence of polymorphism for CAST gene and it could
be a marker to establish association with meat quality traits.