Characterization of Advanced Breeding Lines of Chickpea Under High Temperature Condition Through Morphological and Molecular Markers
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Date
2013
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JNKVV
Abstract
ABSTRACT
Present investigation entitled “Characterization of advanced breeding lines of chickpea under high temperature condition through morphological and molecular markers” was aimed at identification of phenological and morpholological traits influencing seed yield. The experimental material consisted of 100 genotypes grown in randomized complete block design in the All India Co-ordinated Research Project on Chickpea at Seed Breeding Farm, Department of Plant Breeding and Genetics, JNKVV, Jabalpur during Rabi 2012-13. Observations were recorded on eight visual characters and thirteen yield attributing characters for characterization of genotypes.
High heritability coupled with high genetic advance as percentage of mean were recorded for 100- seed weight, seed yield per plant, number of effective pods per plant, harvest index and total number of pods per plant.
The correlation and path coefficient analysis revealed that the traits days to 50% flowering, seeds per pod, number of effective pods per plant, 100-seed weight, number of primary branches and days to maturity showed positive and significant correlation with seed yield per plant as well as direct effect on seed yield. Thus, these traits might be considered for selecting the high yielding genotypes suitable for late planting.
On the basis of D2, 100 genotypes were grouped into 16 clusters. Maximum number of genotypes were recorded in cluster I, followed by cluster II, cluster V and cluster VI, whereas, cluster IV, cluster VII, cluster VIII, cluster IX, cluster X, cluster XI and cluster XII, cluster XIII, cluster XIV , cluster XV and cluster XVI had only one genotype in each.
On the basis of inter-cluster distances, high inter cluster divergence was observed between cluster IX and cluster XV, followed by cluster X and XV, cluster VII and XV, cluster VIII and XV and cluster XI and XV.
On the basis of phenological and yield attributing traits, genotypes ICCV 93014, ICCV 96317, ICCV 01302 and ICCV 063301 were found superior. Theses promising lines can be used as donor for earliness and improvement in yield in hybridization programme.
For estimating genetic diversity at molecular level, eleven SSR markers were generated using dendrogram by neighbor-joining method implemented in Power Marker version 3.25. All the genotypes were grouped into three major groups (clusters). First major group consisted four genotypes (ICCV 93025, ICCV 93024, ICCV 03401 and ICCV 95315), second major group consisted 2 genotypes (ICCV 03405 and ICCV 01306). Maximum numbers of genotype i.e. 94 are present in third group. These major groups were further subdivided into several sub groups.
Present study revealed that the 100 genotypes that are morphologically very much distinct, but at molecular level they are very similar to each other. Some of the genotypes i.e., ICCV 01306 and ICCV 03405 are closely related to each other at both levels. Genotypes ICCV 95315, ICCV 93024 and ICCV 93025 are distinct from each other at both level, hence they might be further used in hybridization programme for generation of superior segregants for heat tolerance.