GELATIN ZYMOGRAPHY OF MATRIX METALLOPROTEINASES IN NEAT SEMEN OF TELLICHERRY GOAT
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Date
2019-12
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Abstract
The present study was carried out to ascertain the presence of Matrix Metalloproteinases (MMPs) enzyme
activity in neat semen samples of breed of Tellicherry goat by gelatin zymography. In gelatin zymography, the
presence of four distinct bands at 135, 125, 92 and 82 kDa of MMP-9 and two bands at 72 and 45kDa of MMP-
2 were observed in all the samples. The catalytically active lower molecular weight MMPs were observed
around 45kDa. The activity of MMP-2 was 3-4 times higher than that of MMP-9. Further, it was inferred that
purification and characterization of the species specific enzymes and their effect on semen samples could
be correlated to use them as an additive in frozen semen samples, as MMP played extensive role in various
physiological processes viz., fertilization, ovulation, milk secretion.
Description
TNV_35thVCRI_CA_Dec-2019_SRSO-21
Keywords
Veterinary Science