GENETIC VARIABILITY FOR GAMMA RAYS INDUCED MUTAGENESIS FOR PRODUCTIVITY PER SE TRAITS AND MALE STERILITY IN ADVANCE GENERATIONS OF RAGI (Eleusine coracana L. Gaertn.)
Loading...
Files
Date
2019-07-22
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE
Abstract
important traits. Therefore, an investigation was aimed to study the gamma rays induced
mutagenesis for male sterility and productivity traits in ragi. M3 seeds suspected for
partial male sterility of two irradiated genotypes, GPU 28 with 3 doses (300,400,500 Gy)
and KMR 204 with 2 doses (300,400 Gy) were used in present study. Screening for
pollen sterility was done using acetocarmine test in M3 generation and observations were
recorded on ten quantitative traits (except for sterile plants). Seventy six partial sterile
mutants were identified having above 70% pollen sterility, were forwarded for evaluation
of progenies in two experiments. In experiment (a) Sixty three (31 from M3 –GPU 28 and
32 from M3 –KMR 204) were evaluated in progenies phenotypically and 43 progenies
were non segregating (fertile), 20 were segregating for fertility in M4 of GPU 28 and
KMR 204. In experiment (b) a total of 13 partial sterile mutants (6 from M3 –GPU 28 and
7 from M3 –KMR 204) were evaluated in M4 and found 9 mutants with both male and
female sterility. Fifty eight mutants (31 from M3 –GPU 28 and 27 from M3 –KMR 204)
were advanced from M3 to M4 for the evaluation for yield. Ten M4 families (5 from M4 –
GPU 28 and 5 from M4 –KMR 204) and 20 individual mutants (11 from M4 –GPU 28 and
9 from M4 –KMR 204) were found in M4 generation having higher seed yield than
checks.