BIOCHEMICAL CHARACTERIZATION TO ASSESS GENETIC DIVERSITY IN WILD POMEGRANATE OF H.P.
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Date
2009
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Abstract
ABSTRACT
The present investigation on “Biochemical characterization to assess genetic
diversity in wild pomegranate of H.P” was carried out using randomly amplified
polymorphic DNA (RAPD) markers. On the basis of morphological studies collection
Shoghi-2 of Shoghi site was seen to possess maximum petiole length and vitamin C
content in aril juice. Genomic DNA was isolated from young and green leaves of 24
collections of six sites of wild pomegranate using CTAB method (Doyle and Doyle,1987)
with slight modifications. Genetic variation was studied using 25 random decamer
primers, out of these only 19 primers produced polymorphism. Total number of bands
amplified were 142, out of which 116 were polymorphic and 19 were specific RAPD
markers. The amplified fragments ranged in size from 178-3895 bp and percentage of
total polymorphism band was 70.All the 24 collections of the six sites were distinguished
with the combination of 19 primers selected in this study. Similarity matrix was
constructed using Dice and Jaccard coefficient. It ranged from 0.42 -0.91 (Jaccard
coefficient) and 0.60-0.92 (Dice coefficient). Low similarity value was obtained between
Rajgarh-3 and Kandaghat-2 and high similarity was between Badiyal-2 and Shoghi-4.
Dendrogram was constructed by using UPGMA method for the clustering for all the
collections of wild pomegranate. All the collections were grouped together except for
Rajgarh-3, which formed another cluster. Relationship between individual site and its
collections were not clear from the dendrogram, for this purpose individual dendrogram
between single site and its collections was constructed. From the data obtained in this
study it can be concluded that RAPD studies can be useful in breeding programmes
allowing the identification of different collections and assessing the genetic similarity
among different collections of wild pomegranate which would facilitate their use as
identified genetic stock in future breeding programmes.