GENETIC DIVERSITY OF ROOT NODULATING BACTERIA ASSOCIATED WITH PEA IN DIFFERENT AGRO-CLIMATIC ZONES OF HIMACHAL PRADESH
Loading...
Date
2018-04
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UHF,NAUNI
Abstract
ABSTRACT
The present investigations were conducted to determine the diversity of rhizobia with multiple
plant growth promoting traits by amplified ribosomal DNA restriction analysis (ARDRA). A total of
72 isolates associated with pea were authenticated as Rhizobium spp. on the basis of different
authentication tests, viz. congo red test, bromothymol blue test, growth in Hofer’s alkaline broth,
ketolactose medium, glucose peptone agar and plant infection test. Out of 72 rhizobial isolates, sixty
were P-solubilizers, forty eight were siderophore producers and only three were HCN producers.
Forty six showed antagonism against Fusarium ultimum, forty against Pythium aphanidermatum and
eighteen against Rhizoctonia solani. On the basis of different banding pattern generated through
ARDRA analysis using Alu I and Msp I, 17 isolates from agro-climatic zone-I were grouped into three
major phylotypes at 56.6 per cent similarity level, 21 isolates from agro-climatic zone-II were
segregated into six major phylotypes at 55 per cent similarity level, 21 isolates from agro-climatic
zone-III were grouped into seven major phylotypes at 55 per cent similarity level and 13 isolates from
agro-climatic zone-IV were grouped into five major phylotypes at 32 per cent similarity level. Among
21 sequenced rhizobial isolates, 9 isolates (HS1, UR9, SP5, SS1, KB2, CS3, SJ6, KC2 and KC3) were
Rhizobium spp., 5 isolates (KN2, MJ4, SN2, SK2 and SS3) were R. leguminosarum and 7 isolates (BD1,
SNE2, SJ1, SJ8, LK2, LT2 and KS1) were R. leguminosarum bv. viciae. The maximum P-solubilization
(305.40 μg/ml), siderophore production (84.32%) was recorded for R. leguminosarum bv. viciae strain
LK2, Maximum IAA production (82.75) μg/ml was noted for R. leguminosarum bv. viciae SJ8. Seed
bacterization with R. leguminosarum bv. viciae strain LK2 has significantly increased shoot, root
length, number of nodules and available NPK over uninoculated control by 27, 76, 57, 15, 25 and 12
per cent, respectively.
Description
Keywords
null