SUITABILITY OF SALINE-ADENINE-GLUCOSE MANNITOL ADDITIVE IN CITRATE-PHOSPHATE DEXTROSE-ADENINE FOR STORING PACKED RED BLOOD CELLS OF GOATS
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Date
2021-01-21
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR
Abstract
In veterinary transfusion medicine, studies related to farm animal transfusions are
scarce. Due to the rise in emotional and commercial value, need of transfusion in this
area is increasing day by day. The present study entitled ‘Suitability of saline-adenine glucose-mannitol additive in citrate-phosphate-dextrose-adenine for storing packed red
blood cells of goats’ envisages the identification of a suitable storage media for goat
blood upto 42 days.
Ten apparently healthy goats weighing 35 to 60 kg within an age range of 4 to
8 years maintained at University Goat and Sheep Farm, Mannuthy and other organized
farms in Thrissur district were selected for the study. Whole blood units were collected
from the selected animals using commercially available CPDA-SAGM blood bags
under aseptic conditions. After centrifugation of each unit (350ml), plasma was
separated and SAGM were added to pRBCs and stored at 4 ± 20C in dedicated
refrigerator upto 42 days. For analysing the parameters, samples were collected from
the bag every two weeks from the day of collection to day 42. Light and scanning
electron microscopic (SEM) studies were carried out on day 0, 28 and 42 of storage.
Biochemical and haematological parameters, RBC haemolysis assessment,
morphological variation and cultural studies of stored blood was carried out.
The pH of the stored blood remained stable whereas glucose showed a
significant reduction. Potassium, malondialdehyde and reduced glutathione increased
throughout the storage. Haemoglobin and volume of packed red cells did not show any
significant change during storage. Haemolysis of RBCs was analysed using osmotic
fragility test. The mean cell fragility of pRBCs did not show a major change from day
14 to 42 of storage. Anisocytosis and poikilocytosis was recorded in light microscopy.
In SEM only a few acanthocytes and dacrocytes were recorded. Storage related
variations were recorded in the samples as the storage period progressed. The study
suggests that CPDA-SAGM is a suitable storage media for caprine pRBCs.
Description
Thesis Submitted in the partial fulfillment of the requirement for the degree of Master of Veterinary Science in Veterinary Clinical Medicine, Ethics and Jurisprudence