SUITABILITY OF SALINE-ADENINE-GLUCOSE MANNITOL ADDITIVE IN CITRATE-PHOSPHATE DEXTROSE-ADENINE FOR STORING PACKED RED BLOOD CELLS OF GOATS

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dc.contributor.advisorN. Madhavan Unny
dc.contributor.authorANAZ S.R.
dc.date.accessioned2023-01-30T10:48:50Z
dc.date.available2023-01-30T10:48:50Z
dc.date.issued2021-01-21
dc.descriptionThesis Submitted in the partial fulfillment of the requirement for the degree of Master of Veterinary Science in Veterinary Clinical Medicine, Ethics and Jurisprudenceen_US
dc.description.abstractIn veterinary transfusion medicine, studies related to farm animal transfusions are scarce. Due to the rise in emotional and commercial value, need of transfusion in this area is increasing day by day. The present study entitled ‘Suitability of saline-adenine glucose-mannitol additive in citrate-phosphate-dextrose-adenine for storing packed red blood cells of goats’ envisages the identification of a suitable storage media for goat blood upto 42 days. Ten apparently healthy goats weighing 35 to 60 kg within an age range of 4 to 8 years maintained at University Goat and Sheep Farm, Mannuthy and other organized farms in Thrissur district were selected for the study. Whole blood units were collected from the selected animals using commercially available CPDA-SAGM blood bags under aseptic conditions. After centrifugation of each unit (350ml), plasma was separated and SAGM were added to pRBCs and stored at 4 ± 20C in dedicated refrigerator upto 42 days. For analysing the parameters, samples were collected from the bag every two weeks from the day of collection to day 42. Light and scanning electron microscopic (SEM) studies were carried out on day 0, 28 and 42 of storage. Biochemical and haematological parameters, RBC haemolysis assessment, morphological variation and cultural studies of stored blood was carried out. The pH of the stored blood remained stable whereas glucose showed a significant reduction. Potassium, malondialdehyde and reduced glutathione increased throughout the storage. Haemoglobin and volume of packed red cells did not show any significant change during storage. Haemolysis of RBCs was analysed using osmotic fragility test. The mean cell fragility of pRBCs did not show a major change from day 14 to 42 of storage. Anisocytosis and poikilocytosis was recorded in light microscopy. In SEM only a few acanthocytes and dacrocytes were recorded. Storage related variations were recorded in the samples as the storage period progressed. The study suggests that CPDA-SAGM is a suitable storage media for caprine pRBCs.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810192939
dc.keywordsAnaemia in goats , Etiology, Clinical findings, Storage of blood, Citrate Phosphate Dextrose Adenine -1, Biochemical studies , Haematological studies, Estimation of erythrocyte reduced glutathione (GSH), Estimation of erythrocyte malondialdehyde (MDA),en_US
dc.language.isoEnglishen_US
dc.pages112p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSURen_US
dc.research.problemStudy the suitability of saline-adenine-glucose-mannitol (SAGM) additive in citrate- phosphate-dextrose-adenine (CPDA) for storing packed red blood cells (pRBCs) of goatsen_US
dc.subClinical Veterinary Medicine, Ethics and Jurisprudenceen_US
dc.themeSUITABILITY OF SALINE-ADENINE-GLUCOSE-MANNITOL ADDITIVE IN CITRATE-PHOSPHATE-DEXTROSE-ADENINE FOR STORING PACKED RED BLOOD CELLS OF GOATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleSUITABILITY OF SALINE-ADENINE-GLUCOSE MANNITOL ADDITIVE IN CITRATE-PHOSPHATE DEXTROSE-ADENINE FOR STORING PACKED RED BLOOD CELLS OF GOATSen_US
dc.typeThesisen_US
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