ASSESSMENT OF TETRACYCLINE RESIDUE IN CHICKEN MEAT AND ITS IMPACT ON PUBLIC HEALTH
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Date
2022
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Abstract
Antimicrobial agents are commonly used in animal production since 1938. After the discovery of tetracyclines in the early 1940s, they were commercialized with clinical success in the late 1940s to the early 1950s. Due to the intensification of broiler production, the incidence of diseases increases, and hence the use of antimicrobials increased especially tetracyclines, because of its broad spectrum of activity, cost-effectiveness, and easy availability. Antimicrobial residues in livestock products can cause deleterious effects to consumers and also results in antimicrobial resistance. To overcome this, regulatory agencies globally recommend Maximum Residual Limit (MRL) by following strict withdrawal periods.
In the present study, it was proposed to screen chicken meat samples for residues of oxytetracycline (OTC). For residue analysis, a High-Performance Liquid Chromatography (HPLC) method was standardized. The method was validated for recovery, sensitivity and, precision. The method was also compared with an ELISA kit method for the detection of OTC residue. To understand the
impact of residue, resistance to OTC among isolates of E. coli of chicken origin was studied and the minimum inhibitory concentration (MIC) of OTC against the isolates of E. coli was tested. In addition, polymerase chain reaction (PCR) was performed to understand the involvement of three genes tet(C), tet(D), and tet(M), in conferring resistance to OTC in E. coliisolates.