IDENTIFICATION OF GENOMIC REGIONS HARBORING QTLs FOR STAY-GREEN AND RELATED TRAITS IN SORGHUM [Sorghum bicolor{L) Moench]
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Date
2007-08-30
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University of Agricultural Sciences GKVK, Banglore
Abstract
Stay-green feature in cereals, the ability of the plant to retain significant
green leaf area until complete grain maturity, is considered important as it
allows proper grain fill and such genotypes are often drought tolerant. In
sorghum, stay-green feature permits complete grain fill during terminal moisture
stress, enables the plant to resist charcoal rot {stalk rot) disease and produces
quality fodder. Being a quantitative character it is difficult to breed for this
character. Present technology permits construction of detailed genetic map with
the help of molecular markers and to identify genomic regions responsible for
any quantitative trait.
In the present investigation, a recombinant inbred population (226)
derived from N 13 (senescent) x E 36-1 (stay-green) was phenotyped for one
season {rabi 2005-06) and genotyped with 28 polymorphic sorghum E5T-SSR
markers, recently developed in our lab. The genetic data for 32 SSR markers
already mapped using the same population, kindly provided by ICRISAT. India,
was used to anchor the EST-SSR markers on the map. These 32 markers were
distributed on LG1-0, LG2-3, LG3-5, LG4-5, LG5-1, LG6-4, LG7-2, LG8-6, LG9-3
and LG10-3. Seven QTLs were detected at a cut off LOD 2.5. Three of them were
located on linkage group B. Except one QTL on linkage group D, others were
found at new regions, previously not reported. Five QTLs contributed by the
stay-green parent E 36-1 accounted for 33.34 per cent of the phenotypic
variation.
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